Team:UCLA/Notebook/Honeybee Silk/30 July 2015

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Running HoneyBee Protein on SDS PAGE gel

• In the morning I incubated the purified inclusion bodies in a slightly above RT water bath in order to resolubilize the SDS and the protein.
• I then took a 40 ul fraction and put the rest in the 4c room for storage.
• Fasih Asahn taught me how to load a pre-cast SDS PAGE gel with my samples.

1. Put the samples on ice.
2. Establish what sample will go in which lane.
   • From left to right it is F1,S1,F2,F3,S2,S3, Final product, and BSA positive control.