Team:UCLA/Notebook/Honeybee Silk/3 August 2015

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Analyzing SDS PAGE Gel results

• Danny ran the SDS PAGE gel for the honeybee purification protocol on 7/30 and Megan stained the gel for visualization of 7/31.
  • The gel was run, and then stored overnight in destain buffer due to logistical reasons before being stained the next day.
• Here is the gel
  

  Fig. 1 Expected size of product is 40 kDa (lane 7). Lane 8 is BSA protein positive control, and lane 9 is Biorad dual color ladder. From lane 1-9 F1,S1,F2,F3,S2,S3, Final product, BSA positive control, and ladder (see table)
• Refer to 7/29 for more detailed description of when each fraction was taken.