Team:UCLA/Notebook/Honeybee Silk/3 August 2015
Analyzing SDS PAGE Gel results
- Danny ran the SDS PAGE gel for the honeybee purification protocol on 7/30 and Megan stained the gel for visualization of 7/31.
- The gel was run, and then stored overnight in destain buffer due to logistical reasons before being stained the next day.
- Here is the gel
- Refer to 7/29 for more detailed description of when each fraction was taken.
F1 (lane 1) | Full cell lysate using BugBuster lysis system |
---|---|
S1 (lane 2) | Supernatant after spinning down full cell lysate |
F2 (lane 3) | Full cell lysate #2 after resuspending pellet in Bugbuster |
F3 (lane 4) | Full cell lysate #3 after adding lysozyme and DNase |
S2 (lane 5) | Supernatant after first wash step. |
S3 (lane 6) | Supernatant after second wash step |
Final product (lane 7) | Purified inclusion body in SDS |
positive control (lane 8) | BSA positive control |
ladder (lane 9) | BioRad dual color ladder |