Team:UCLA/Notebook/Honeybee Silk/12 August 2015

• HOME
• TEAM
• PROJECTS
  • Silk Genetics
  • Functionalization
  • Materials Processing
  • Protein Cages
• PARTS
  • Team Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
• NOTEBOOKS
  • Spider Silk Genetics
  • Honeybee Silk
  • Recombinant Silk Functionalization
  • Materials Processing
  • Protein Cages
  • UCLA Measurement Interlab Study
• MEETING NOTES
  • General Meetings
  • Coordinator Meetings
  • Advisor Meetings
• ATTRIBUTIONS
• COLLABORATIONS
• HUMAN PRACTICES
• SAFETY
• MEASUREMENT STUDY

Multiple Mini Expressions: Optimizing protein yield

• Today we are testing multiple growth conditions of our bl21 cells for total cell and protein yield. Parameters we are optimizing are iptg concentration, and growth temperature.

IPTG concentration/Temp	37C	30C
0.1 mM
0.5 mM
1.0 mM
2.0 mM