Difference between revisions of "Team:Cambridge-JIC/Tech Specs"
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− | <img src="// | + | <img src="https://static.igem.org/mediawiki/2015/8/88/CamJIC-Flexure.gif" style="height:300px; margin:15px;float:left"> |
<h3>Stage Translation Mechanism</h3> | <h3>Stage Translation Mechanism</h3> | ||
<p>When designing the OpenScope stage, we have taken advantage of the flexibility of the plastic, following the strategy developed by Dr Richard Bowman. The legs of the sample stage extend into horizontal levers in two perpendicular directions. Flexing these 'levers' allows for x,y-translation. The total range of movement is approximately 1cmx1cm. Screws are used to press the livers (with nuts glued to their bottom face). This rigid connection allows for fine control, precise positioning and stability. Plastic 3D-printed caps and smooth gears are added on top of the screws to make it easy for you to move the stage manually. Another alternative is motorizing the stage (for more info, see below).</p> | <p>When designing the OpenScope stage, we have taken advantage of the flexibility of the plastic, following the strategy developed by Dr Richard Bowman. The legs of the sample stage extend into horizontal levers in two perpendicular directions. Flexing these 'levers' allows for x,y-translation. The total range of movement is approximately 1cmx1cm. Screws are used to press the livers (with nuts glued to their bottom face). This rigid connection allows for fine control, precise positioning and stability. Plastic 3D-printed caps and smooth gears are added on top of the screws to make it easy for you to move the stage manually. Another alternative is motorizing the stage (for more info, see below).</p> | ||
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<h3>Movement Precision</h3> | <h3>Movement Precision</h3> | ||
− | <p><img src="//2015.igem.org/wiki/images/3/32/CamJIC-Specs-Motors.jpg" style="height:300px; margin: 5px;float:right">The flexure mechanism allows the stage to move horizontally very smoothly, and all care was taken to provide just as smooth vertical movement. Using screws to control the mechanics provides an extra degree of fine-control. In addition, the microscope can be motorized for complete remote operation (again, through the <a href="https://2015.igem.org/Team:Cambridge-JIC/Webshell" class="blue">WebShell</a>). We used cheap, low-power (operating at 5V) stepper motors, which are still highly accurate: they are geared to 513 steps per revolution, which translates into approx. | + | <p><img src="//2015.igem.org/wiki/images/3/32/CamJIC-Specs-Motors.jpg" style="height:300px; margin: 5px;float:right">The flexure mechanism allows the stage to move horizontally very smoothly, and all care was taken to provide just as smooth vertical movement. Using screws to control the mechanics provides an extra degree of fine-control. In addition, the microscope can be motorized for complete remote operation (again, through the <a href="https://2015.igem.org/Team:Cambridge-JIC/Webshell" class="blue">WebShell</a>). We used cheap, low-power (operating at 5V) stepper motors, which are still highly accurate: they are geared to 513 steps per revolution, which translates into approx. 1.0μm movement of the sample per step along the x- and y-axes. For the detailed analysis, see the document below.</p> |
<center><a class="btn btn-default" href="//2015.igem.org/wiki/images/4/4d/CamJIC-Specs-Movement.pdf" role="button" style="color:#444;border-color:#444">download pdf</a></center> | <center><a class="btn btn-default" href="//2015.igem.org/wiki/images/4/4d/CamJIC-Specs-Movement.pdf" role="button" style="color:#444;border-color:#444">download pdf</a></center> | ||
</div></div></section> | </div></div></section> | ||
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<h3>Strength</h3> | <h3>Strength</h3> | ||
<p><img src="//2015.igem.org/wiki/images/8/84/CamJIC-Instron_1.png" style="height:300px;margin:5px;float:right"></center>Three point bending tests were performed to confirm the strength of the OpenScope stage. The material used to print it - PLA - turns out to be both 4 times stronger and and 13 times stiffer than the alternative material, ABS. We also explored how different orientations of the object during 3D-printing affects its strength, and took this into account when putting together the OpenScope print files. Moreover, it was confirmed that the plastic is not subject to plastic deformation due to the weight-loads that the OpenScope stage will typically have to bear. In short: you can put OpenScope in your backpack and not worry about it getting broken (we tried this, too). The detailed report from the strength testing experiments can be found below.</p> | <p><img src="//2015.igem.org/wiki/images/8/84/CamJIC-Instron_1.png" style="height:300px;margin:5px;float:right"></center>Three point bending tests were performed to confirm the strength of the OpenScope stage. The material used to print it - PLA - turns out to be both 4 times stronger and and 13 times stiffer than the alternative material, ABS. We also explored how different orientations of the object during 3D-printing affects its strength, and took this into account when putting together the OpenScope print files. Moreover, it was confirmed that the plastic is not subject to plastic deformation due to the weight-loads that the OpenScope stage will typically have to bear. In short: you can put OpenScope in your backpack and not worry about it getting broken (we tried this, too). The detailed report from the strength testing experiments can be found below.</p> | ||
− | <center><a class="btn btn-default" href="//2015.igem.org/wiki/images/ | + | <center><a class="btn btn-default" href="//2015.igem.org/wiki/images/3/37/CamJIC-Specs-Materials.pdf" role="button" style="color:#444;border-color:#444">download pdf</a></center> |
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<h3>Electronics</h3> | <h3>Electronics</h3> | ||
<p>The OpenScope is a digital microscope based on open-source elctronics. Raspberry Pi provides the processing power, required to run the <a href="https://2015.igem.org/Team:Cambridge-JIC/Downloads#Software" class="blue">NOOBSCOPE</a> software package, that we created. It enables your OpenScope to stream real-time picture over the WebShell... and much more (Read about this in the <a href="https://2015.igem.org/Team:Cambridge-JIC/Webshell" class="blue">Software</a> section). | <p>The OpenScope is a digital microscope based on open-source elctronics. Raspberry Pi provides the processing power, required to run the <a href="https://2015.igem.org/Team:Cambridge-JIC/Downloads#Software" class="blue">NOOBSCOPE</a> software package, that we created. It enables your OpenScope to stream real-time picture over the WebShell... and much more (Read about this in the <a href="https://2015.igem.org/Team:Cambridge-JIC/Webshell" class="blue">Software</a> section). | ||
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<h3>Affordable Price</h3> | <h3>Affordable Price</h3> | ||
− | <p>The total cost of OpenScope is determined mainly by the price of the electronics used, given that the plastic 3D-printed components round up to £2 (100g of PLA, £20 per kg). You will also definitely need the Raspberry Pi camera(£15-20), the Raspberry Pi itself (£25), an Arduino PCB ( | + | <p>The total cost of OpenScope is determined mainly by the price of the electronics used, given that the plastic 3D-printed components round up to £2 (100g of PLA, £20 per kg). You will also definitely need the Raspberry Pi camera(£15-20), the Raspberry Pi itself (£25), an Arduino PCB (£3-£18) and some LEDs. On top of that are some optional components: motors if you want to control OpenScope remotely and filters if you will be imaging fluorescence. To figure out which components you need and which not, for your own OpenScope, consult our <a href="https://2015.igem.org/Team:Cambridge-JIC/Make_Your_Own" class="blue">Make Your Own</a> page. You can download the full Bill of Materials below. The total price stays in the range £100-150, which is two or more orders of magnitude lower than the price of the commercial lab microscopes.</p> |
− | <center><a class="btn btn-default" href=" | + | <center><a class="btn btn-default" href="//2015.igem.org/wiki/images/d/d0/CamJIC-OpenScope-BOM.pdf" role="button" style="color:#444;border-color:#444">download pdf</a></center> |
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Latest revision as of 16:47, 12 October 2015