Difference between revisions of "Team:Pasteur Paris/Notebook"

Line 202: Line 202:
 
         <div class="row">           
 
         <div class="row">           
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_2">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_2">
             <div class="col-lg-12" id="week_2" style=" background-image:url(https://static.igem.org/mediawiki/2015/1/1c/Week2photo.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_2')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_2" style=" background-image:url(https://static.igem.org/mediawiki/2015/1/1c/Week2photo.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_2')" onmouseout="EfaceHelp()">
 
               Week 2 <p>06/08 - 06/12</p>
 
               Week 2 <p>06/08 - 06/12</p>
 
             </div>
 
             </div>
Line 215: Line 215:
 
       <div class="row">
 
       <div class="row">
 
         <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_3">
 
         <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_3">
         <div class="col-lg-12" id="week_3" style=" background-image:url(https://static.igem.org/mediawiki/2015/6/6d/Week3.JPG)" onmouseover="Help('', 'week_3')" onmouseout="EfaceHelp()">  
+
         <div class="col-lg-12 week" id="week_3" style=" background-image:url(https://static.igem.org/mediawiki/2015/6/6d/Week3.JPG)" onmouseover="Help('', 'week_3')" onmouseout="EfaceHelp()">  
 
               Week 3 <p>06/15 - 06/19</p>
 
               Week 3 <p>06/15 - 06/19</p>
 
             </div>
 
             </div>
Line 228: Line 228:
 
         <div class="row">           
 
         <div class="row">           
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_4">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_4">
             <div class="col-lg-12" id="week_4" style=" background-image:url(https://static.igem.org/mediawiki/2015/6/69/Week2test.JPG)" onmouseover="Help('We recieved bacteria harboring the Biobrick K808026 and so we cultured the in order to perform a plasmid extraction.', 'week_4')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_4" style=" background-image:url(https://static.igem.org/mediawiki/2015/6/69/Week2test.JPG)" onmouseover="Help('We recieved bacteria harboring the Biobrick K808026 and so we cultured the in order to perform a plasmid extraction.', 'week_4')" onmouseout="EfaceHelp()">
 
               Week 4 <p>06/22 - 06/26</p>
 
               Week 4 <p>06/22 - 06/26</p>
 
             </div>
 
             </div>
Line 241: Line 241:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_5">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_5">
             <div class="col-lg-12" id="week_5" style=" background-image:url(https://static.igem.org/mediawiki/2015/b/b6/Week12.JPG)" onmouseover="Help('We transformed each Biobrick that we had received (K1095000) and verified that we had recieved the right biobrick by digesting the plasmid and performing an electrophoresis on agarose gel and we did a plasmid purification. We performed stab cultures (BBa_808007, 808010, 808011, 808012, 808013, 808014, 316003, 936000, 936013).', 'week_5')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_5" style=" background-image:url(https://static.igem.org/mediawiki/2015/b/b6/Week12.JPG)" onmouseover="Help('We transformed each Biobrick that we had received (K1095000) and verified that we had recieved the right biobrick by digesting the plasmid and performing an electrophoresis on agarose gel and we did a plasmid purification. We performed stab cultures (BBa_808007, 808010, 808011, 808012, 808013, 808014, 316003, 936000, 936013).', 'week_5')" onmouseout="EfaceHelp()">
 
               Week 5 <p>06/29 - 07/03</p>
 
               Week 5 <p>06/29 - 07/03</p>
 
             </div>
 
             </div>
Line 254: Line 254:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_6">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_6">
             <div class="col-lg-12" id="week_6" style=" background-image:url(https://static.igem.org/mediawiki/2015/e/eb/Week6.JPG)" onmouseover="Help('We prepared oligonucleotides in order to send then for sequencing at MWG Eurofins. We also did another round of stab cultures.', 'week_6')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_6" style=" background-image:url(https://static.igem.org/mediawiki/2015/e/eb/Week6.JPG)" onmouseover="Help('We prepared oligonucleotides in order to send then for sequencing at MWG Eurofins. We also did another round of stab cultures.', 'week_6')" onmouseout="EfaceHelp()">
 
               Week 6 <p>07/06 - 07/10</p>
 
               Week 6 <p>07/06 - 07/10</p>
 
             </div>
 
             </div>
Line 267: Line 267:
 
         <div class="row">           
 
         <div class="row">           
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_7">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_7">
             <div class="col-lg-12" id="week_7" style=" background-image:url(https://static.igem.org/mediawiki/2015/0/07/Week7.JPG)" onmouseover="Help('We performed our first PCR amplification of the genes that we would use for our Gibson Assembly and we checked if the PCR was done correctly by performing an electrophoresis on agarose.', 'week_7')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_7" style=" background-image:url(https://static.igem.org/mediawiki/2015/0/07/Week7.JPG)" onmouseover="Help('We performed our first PCR amplification of the genes that we would use for our Gibson Assembly and we checked if the PCR was done correctly by performing an electrophoresis on agarose.', 'week_7')" onmouseout="EfaceHelp()">
 
               Week 7 <p>06/13 - 07/17</p>
 
               Week 7 <p>06/13 - 07/17</p>
 
             </div>
 
             </div>
Line 280: Line 280:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_8">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_8">
             <div class="col-lg-12" id="week_8" style=" background-image:url(https://static.igem.org/mediawiki/2015/8/88/Week8.JPG)" onmouseover="Help('We performed stab cultures on the biobricks that we already had, in order to store them.', 'week_8')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_8" style=" background-image:url(https://static.igem.org/mediawiki/2015/8/88/Week8.JPG)" onmouseover="Help('We performed stab cultures on the biobricks that we already had, in order to store them.', 'week_8')" onmouseout="EfaceHelp()">
 
               Week 8 <p>07/20 - 07/24</p>
 
               Week 8 <p>07/20 - 07/24</p>
 
             </div>
 
             </div>
Line 293: Line 293:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_9">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_9">
             <div class="col-lg-12" id="week_9" style=" background-image:url(https://static.igem.org/mediawiki/2015/9/9e/DSCN1936.JPG)" onmouseover="Help('We did a bacterial culture of DH5-alpha bacteria harboring the 808030 Biobrick.', 'week_9')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_9" style=" background-image:url(https://static.igem.org/mediawiki/2015/9/9e/DSCN1936.JPG)" onmouseover="Help('We did a bacterial culture of DH5-alpha bacteria harboring the 808030 Biobrick.', 'week_9')" onmouseout="EfaceHelp()">
 
               Week 9 <p>07/27 - 07/31</p>
 
               Week 9 <p>07/27 - 07/31</p>
 
             </div>
 
             </div>
Line 306: Line 306:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_10">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_10">
             <div class="col-lg-12" id="week_10" style=" background-image:url(https://static.igem.org/mediawiki/2015/4/48/Week10.JPG)" onmouseover="Help('The week was spent testing the toxicity, solubility and use of TPA as a carbon source by the bacteria BAP1. In order to do this, we prepared different types of medium. This week, we also purified and ligated BBa_808030 and the plasmid Backbone pDG011 and checking if the ligation had worked. We also prepared the fragments for the Gibson assembly through PCR Amplification or ligation, depending on the fragment.', 'week_10')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_10" style=" background-image:url(https://static.igem.org/mediawiki/2015/4/48/Week10.JPG)" onmouseover="Help('The week was spent testing the toxicity, solubility and use of TPA as a carbon source by the bacteria BAP1. In order to do this, we prepared different types of medium. This week, we also purified and ligated BBa_808030 and the plasmid Backbone pDG011 and checking if the ligation had worked. We also prepared the fragments for the Gibson assembly through PCR Amplification or ligation, depending on the fragment.', 'week_10')" onmouseout="EfaceHelp()">
 
               Week 10 <p>08/03 - 08/07</p>
 
               Week 10 <p>08/03 - 08/07</p>
 
             </div>
 
             </div>
Line 319: Line 319:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_11">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_11">
             <div class="col-lg-12" id="week_11" style=" background-image:url(https://static.igem.org/mediawiki/2015/2/2c/Week11.JPG)" onmouseover="Help('The Gibson assembly of cluster 2 was performed and we performed a PCR amplification on it. Other fragments needed for the Gibson assembly of the other clusters were prepared. The transformation of the plasmid pDG011 in DH5-alpha cells was performed. We did an electrophoresis on BBa_K808030 and pDG011 in order to perform a gel purification. We also did a plasmid purification of the plasmid pDG011.', 'week_11')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_11" style=" background-image:url(https://static.igem.org/mediawiki/2015/2/2c/Week11.JPG)" onmouseover="Help('The Gibson assembly of cluster 2 was performed and we performed a PCR amplification on it. Other fragments needed for the Gibson assembly of the other clusters were prepared. The transformation of the plasmid pDG011 in DH5-alpha cells was performed. We did an electrophoresis on BBa_K808030 and pDG011 in order to perform a gel purification. We also did a plasmid purification of the plasmid pDG011.', 'week_11')" onmouseout="EfaceHelp()">
 
               Week 11 <p>08/10 - 08/14</p>
 
               Week 11 <p>08/10 - 08/14</p>
 
             </div>
 
             </div>
Line 332: Line 332:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_12">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_12">
             <div class="col-lg-12" id="week_12" style=" background-image:url(https://static.igem.org/mediawiki/2015/b/b2/Week5.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_12')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_12" style=" background-image:url(https://static.igem.org/mediawiki/2015/b/b2/Week5.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_12')" onmouseout="EfaceHelp()">
 
               Week 12 <p>08/17 - 08/21</p>
 
               Week 12 <p>08/17 - 08/21</p>
 
             </div>
 
             </div>
Line 345: Line 345:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_13">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_13">
             <div class="col-lg-12" id="week_13" style=" background-image:url(https://static.igem.org/mediawiki/2015/c/ca/Week13.jpeg)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_13')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_13" style=" background-image:url(https://static.igem.org/mediawiki/2015/c/ca/Week13.jpeg)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_13')" onmouseout="EfaceHelp()">
 
               Week 13 <p>08/24 - 08/28</p>
 
               Week 13 <p>08/24 - 08/28</p>
 
             </div>
 
             </div>
Line 358: Line 358:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_14">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_14">
             <div class="col-lg-12" id="week_14" style=" background-image:url(https://static.igem.org/mediawiki/2015/a/aa/42f1c36c14288e405732346a2810579b.jpg)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_14')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_14" style=" background-image:url(https://static.igem.org/mediawiki/2015/a/aa/42f1c36c14288e405732346a2810579b.jpg)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_14')" onmouseout="EfaceHelp()">
 
               Week 14 <p>08/31 - 09/04</p>
 
               Week 14 <p>08/31 - 09/04</p>
 
             </div>
 
             </div>
Line 371: Line 371:
 
       <div class="row">
 
       <div class="row">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_15">
 
           <a href="https://2015.igem.org/Team:Pasteur_Paris/Week_15">
             <div class="col-lg-12" id="week_15" style=" background-image:url(https://static.igem.org/mediawiki/2015/0/02/Week15.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_15')" onmouseout="EfaceHelp()">
+
             <div class="col-lg-12 week" id="week_15" style=" background-image:url(https://static.igem.org/mediawiki/2015/0/02/Week15.JPG)" onmouseover="Help('We verified that the plasmid purification was done properly by measuring the concentration of each Biobrick and we verified that the insert caried was the one we expected through a digestion and an electrophoresis.', 'week_15')" onmouseout="EfaceHelp()">
 
               Week 15 <p>09/07 - 09/11</p>
 
               Week 15 <p>09/07 - 09/11</p>
 
             </div>
 
             </div>

Revision as of 14:23, 16 September 2015

Notebook

Search for a content: