Difference between revisions of "Team:Pasteur Paris/Week 12"
Line 226: | Line 226: | ||
<br/> | <br/> | ||
− | < | + | <center><h3><em>DNA concentration assay</em></h3></center></br> |
<table border="1" cellspacing="0" cellpadding="0" width="678"> | <table border="1" cellspacing="0" cellpadding="0" width="678"> | ||
Line 287: | Line 287: | ||
− | <h3 | + | <center><h3><em>Gibson Assembly</em></h3></center></br> |
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>Enzymatic digest of K936011, K936023 and K1392932</li> | <li>Enzymatic digest of K936011, K936023 and K1392932</li> | ||
Line 296: | Line 296: | ||
<br /> | <br /> | ||
− | <h3 | + | <center><h3><em>Cluster 4A Gibson assembly</em></h3></center></br> |
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>Gibson Assembly</li> | <li>Gibson Assembly</li> | ||
Line 303: | Line 303: | ||
</ul> | </ul> | ||
<br/> | <br/> | ||
− | < | + | <center><h3><em>Gel plan</em></h3></center></br> |
<table border="1" cellspacing="0" cellpadding="0" width="642"> | <table border="1" cellspacing="0" cellpadding="0" width="642"> |
Revision as of 23:34, 3 October 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 12 NB-Esterase Assay
pNP-Assay
Gibson AssemblyOD measurement of Cluster 4:
Gel plan:
DNA concentration assay
Gibson Assembly
Cluster 4A Gibson assembly
Gel plan
PCR amplification of Biobricks K808007, K808030, K936011, K936023, K1392932, K316003
The PCR worked for most of our Biobricks with the exception of the biobrick K808030. DNA concentration measurement
PCR amplification of pSB1C3
PCR amplification of pSB1C3
Cycles:
PCR amplification using Takara Ex TaqPCR amplification of Biobricks K808030Gel Plan:
PCR amplification of pSB1C3PCR amplification of pSB1C3
Gel Migration on a 1% Agarose GelEnzymatic digest of BBa_K808030 Yeast AssemblyThe bacteria did not grow because the wrong antibiotic was chosen: BBa_J61047 was in pSB1A2 and not pSB1C3.
PCR Amplification of the BBa_J61047 using Phusion Polymerase Master Mix.TPA solubility :^ |