Difference between revisions of "Team:Pasteur Paris/Week 12"
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<li>PCR amplification of cluster 4A Assembly.</li> | <li>PCR amplification of cluster 4A Assembly.</li> | ||
<li>Gel migration on 0.8% agarose gel.</li> | <li>Gel migration on 0.8% agarose gel.</li> | ||
+ | <li>Gel plan</em></li> | ||
</ul> | </ul> | ||
− | + | </br> | |
− | + | ||
<table border="1" cellspacing="0" cellpadding="0" width="642"> | <table border="1" cellspacing="0" cellpadding="0" width="642"> | ||
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</table> | </table> | ||
<br/> | <br/> | ||
− | <h3 | + | |
+ | <center><h3><em>PCR amplification of Biobricks BBa_K808007, BBa_K808030, BBa_K936011, BBa_K936023, BBa_K1392932, BBa_K316003</em></h3></center></br> | ||
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>PCR amplification using Phusion polymerase</li> | <li>PCR amplification using Phusion polymerase</li> | ||
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</table> | </table> | ||
<br /> | <br /> | ||
− | + | <center><h3><em>PCR amplification of pSB1C3</em></h3></center></br> | |
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>PCR amplification using Phusion Polymerase.</li> | <li>PCR amplification using Phusion Polymerase.</li> | ||
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</table> | </table> | ||
<br/> | <br/> | ||
− | <h3 | + | |
+ | <center><h3><em>PCR amplification of pSB1C3</em></h3></center></br> | ||
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>PCR amplification using Takara Ex Taq Polymerase testing different annealing temperatures.</li> | <li>PCR amplification using Takara Ex Taq Polymerase testing different annealing temperatures.</li> | ||
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<br/> | <br/> | ||
<br/> | <br/> | ||
− | <h3 | + | |
+ | <center><h3><em>PCR amplification using Takara Ex Taq</em></h3></center></br> | ||
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>Gel Migration on a 1% agarose gel.</li> | <li>Gel Migration on a 1% agarose gel.</li> | ||
</ul> | </ul> | ||
− | <h3 | + | <center><h3><em>PCR amplification of Biobricks K808030</em></h3></center></br> |
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>PCR Amplification using Takara Ex Taq Polymerase.</li> | <li>PCR Amplification using Takara Ex Taq Polymerase.</li> | ||
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<br /> | <br /> | ||
<br /> | <br /> | ||
− | <h3 | + | <center><h3><em>PCR amplification of pSB1C3</em></h3></center></br> |
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>PCR amplification using Takara Ex Taq Polymerase testing different annealing temperatures.</li> | <li>PCR amplification using Takara Ex Taq Polymerase testing different annealing temperatures.</li> | ||
Line 562: | Line 565: | ||
</ul> | </ul> | ||
− | <h3 | + | <center><h3><em>PCR amplification of pSB1C3</em></h3></center></br> |
<ul style="text-align:left"> | <ul style="text-align:left"> | ||
<li>PCR amplification using Takara Ex Taq Polymerase testing 8 annealing temperatures.</li> | <li>PCR amplification using Takara Ex Taq Polymerase testing 8 annealing temperatures.</li> |
Revision as of 23:38, 3 October 2015
Week 1Week 2Week 3Week 4Week 5Week 6Week 7Week 8Week 9Week 10Week 11Week 12Week 13Week 14Week 15 |
Week 12 NB-Esterase Assay
pNP-Assay
Gibson AssemblyOD measurement of Cluster 4:
Gel plan:
DNA concentration assay
Gibson Assembly
Cluster 4A Gibson assembly
PCR amplification of Biobricks BBa_K808007, BBa_K808030, BBa_K936011, BBa_K936023, BBa_K1392932, BBa_K316003
The PCR worked for most of our Biobricks with the exception of the biobrick K808030. DNA concentration measurement
PCR amplification of pSB1C3
PCR amplification of pSB1C3
Cycles:
PCR amplification using Takara Ex TaqPCR amplification of Biobricks K808030Gel Plan:
PCR amplification of pSB1C3PCR amplification of pSB1C3
Gel Migration on a 1% Agarose GelEnzymatic digest of BBa_K808030 Yeast AssemblyThe bacteria did not grow because the wrong antibiotic was chosen: BBa_J61047 was in pSB1A2 and not pSB1C3.
PCR Amplification of the BBa_J61047 using Phusion Polymerase Master Mix.TPA solubility :^ |