Team:UCLA/Notebook/Honeybee Silk/11 August 2015

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8/11

Protein Yield

• Yield from 8/7 silk purification
• BCA assay
  • Based on the interpolated Absorbencies from the BCA assay the concentration of our 8/7 silk is approximately 0.78 mg/ml in around 12 ml of solution, giving a conservative 9 total mg of protein for a 300 ml culture.
• This is an improvement over the 7/29 purification where we got around 1.6 mg/ml in only 0.75 ml of solution from a 100 ml culture.