Team:Czech Republic/Parts

Parts

Description

We relied heavily on two promoters from the Registry (pGAL1 and pCUP1), and so we extensively characterized them both and submitted the results to the Registry as a part of the Gold medal requirements.

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Basic Parts

Several Basic parts were constructed during the summer. We contributed to the Registry with 8 different basic parts, ready to use in Saccharomyces cerevisiae. Function of all these parts was experimentally validated.

We sent 4 different mating pheromones:

  • Mating pheromone (S. cerevisiae) - [http://parts.igem.org/Part:BBa_K1690000 BBa_K1690000]
  • Mating pheromone (C. albicans) - [http://parts.igem.org/Part:BBa_K1690001 BBa_K1690001]
  • Mating pheromone (C. tropicalis) - [http://parts.igem.org/Part:BBa_K1690002 BBa_K1690002]
  • Mating pheromone (L. elongisporus) - [http://parts.igem.org/Part:BBa_K1690003 BBa_K1690003]

And with the sequences of 2 different pheromone receptors:

  • Pheromone receptor (C. albicans) - [http://parts.igem.org/Part:BBa_K1690004 BBa_K1690004]
  • Pheromone receptor (C. parapsilosis) - [http://parts.igem.org/Part:BBa_K1690005 BBa_K1690005]

The BioBricks above were constructed by Module 2, where you can find details of their construction and function.

Also, 3 different reporters ready to use in Saccharomyces were prepared and sent:

  • pADH1_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690006 BBa_K1690006]
  • pasCYC1v3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690007 BBa_K1690007]
  • pTv3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690008 BBa_K1690008]

These BioBricks were constructed by Module 1, where you can find details of their construction and function.

Part Collection

Our submitted parts can be divided into two separate collections:

Signals & Receptors

  • Mating pheromone (S. cerevisiae) - [http://parts.igem.org/Part:BBa_K1690000 BBa_K1690000]
  • Mating pheromone (C. albicans) - [http://parts.igem.org/Part:BBa_K1690001 BBa_K1690001]
  • Mating pheromone (C. tropicalis) - [http://parts.igem.org/Part:BBa_K1690002 BBa_K1690002]
  • Mating pheromone (L. elongisporus) - [http://parts.igem.org/Part:BBa_K1690003 BBa_K1690003]
  • Pheromone receptor (C. albicans) - [http://parts.igem.org/Part:BBa_K1690004 BBa_K1690004]
  • Pheromone receptor (C. parapsilosis) - [http://parts.igem.org/Part:BBa_K1690005 BBa_K1690005]

Yeast reporters

  • pADH1_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690006 BBa_K1690006]
    • GFP under the control of constitutive pADH1 promoter
  • pasCYC1v3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690007 BBa_K1690007]
    • GFP under the control of a MATa specific constitutive pasCYC1v3 promoter
  • pTv3_GFP (Yeast reporter) - [http://parts.igem.org/Part:BBa_K1690008 BBa_K1690008]
    • GFP under the control of repressible pTet promoter