Team:Czech Republic/Achievements

Achievements

Medal Criteria

Gold Medal

At least two of the following goals:

✓ Demonstrate a functional prototype of your project. Your prototype can derive from a previous project (that was not demonstrated to work) by your team or by another team. Show this system working under real-world conditions that you simulate in the lab.

The functional prototype is fully described on our Design page.

✓ Improve the function OR characterization of a previously existing BioBrick Part or Device (created by another team, or by your own team in in a previous year of iGEM), and enter this information in the part's page on the Registry.

We significantly improved the characterization of two inducible promoters in the Registry - the pCUP1 promoter (can be found as BBa_K945002) and the pGAL1 promoter (can be found as BBa_J63006).

✓ Help any registered iGEM team from a high-school, different track, another university, or institution in a significant way by, for example, mentoring a new team, characterizing a part, debugging a construct, modeling/simulating their system or helping validate a software/hardware solution to a synbio problem.

We helped Team Chalmers Gothenburg model and simulate their system modules comprising a kill switch and a contaminant reporter. We also cooperated in a Multinational GMO survey.

✓ Choose one of these two options:
  1. Expand on your silver medal Human Practices activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.
  2. Demonstrate an innovative Human Practices activity that relates to your project (this typically involves educational, public engagement, and/or public perception activities; see the Human Practices Hub for information and examples of innovative activities from previous teams).

We integrated several ideas from conversations and interviews with two experts in CTC diagnosis. As recommended we tested agglutination in microfluidic chips instead of tubes. Also, as was suggested, for signalling orthogonality tests in Module 2, we used GFP as the output signal instead of agglutination to make the result more obvious. See our Project page for more details.

Silver Medal

All of the following goals:

✓ Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization of this part in the Main Page section of the Registry entry for that Part/Device. This working part must be different from the part you documented in Bronze medal criterion #6.

We validated the functions of all parts we submitted to the Registry. As an example, you can check out our yeast pADH1_GFP reporter. The results can be viewed in the Registry.

✓ Submit this new part to the iGEM Parts Registry. This part must be different from the part you documented in Bronze medal criterion #6. (Submissions must adhere to the iGEM Registry guidelines.)

We submitted this part as BBa_K1690006.

✓ iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. We refer to these activities as Human Practices in iGEM. Demonstrate how your team has identified, investigated and addressed one or more of these issues in the context of your project.

We addressed these issues on our Human Practices page (mainly in the interview with the CTC diagnosis specialists).

Bronze Medal

All of the following goals:

✓ Register for iGEM, have a great summer, and attend the Giant Jamboree.

We are registered!.

✓ Complete the Judging form.

The Judging form was completed.

✓ Create and share a Description of the team's project using the iGEM wiki, and document the team's parts using the Registry of Standard Biological Parts.

Team wiki contains the description of our project and all our parts were documented in the Registry.

✓ Present a poster and a talk at the iGEM Giant Jamboree.

We are going to the Giant Jamboree with a poster and a talk ready.

✓ Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.

This page on our team wiki is up and running.

✓ Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry.

We submitted and documented several parts, for example BBa_K1690000 (the BioBrick numbers we used range from BBa_K1690000 to BBa_K1690008). The documentation can be found in the Registry or at our wiki.

Goals

The IOD band is a project built on system level integration. At the outset, it seemed like an impossible goal for a single iGEM project especially as there are no precedents for many of the system parts. Take a look how we divided the project into specific goals that we also achieved!

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