Team:UCLA/Notebook/Honeybee Silk/17 August 2015
Redo of Ligation of psb1c3+T7 promoter and Silk+GFP
Component | Volume (out of 20uL) |
---|---|
T4 Ligase Reaction Buffer | 2 ul |
Backbone (psb1c3+t7 promoter) | 0.8uL (50 ng) |
Insert (silk + spyC) | 1.5 ul (146 ng) |
H20 | 14.7 ul |
Ligase | 1uL |
- Program = 27C for 1 hour, heat inactivate for 10 minutes at 65C.
SDS PAGE: Determining Protein Quality
- Today we are testing multiple growth conditions of our bl