Team:UCLA/Notebook/Honeybee Silk/17 August 2015

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  • Functionalization
  • Materials Processing
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  • Spider Silk Genetics
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  • UCLA Measurement Interlab Study
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Redo of Ligation of psb1c3+T7 promoter and Silk+GFP

Component	Volume (out of 20uL)
T4 Ligase Reaction Buffer	2 ul
Backbone (psb1c3+t7 promoter)	0.8uL (50 ng)
Insert (silk + spyC)	1.5 ul (146 ng)
H20	14.7 ul
Ligase	1uL

• Program = 27C for 1 hour, heat inactivate for 10 minutes at 65C.

SDS PAGE: Determining Protein Quality

• Today we are testing multiple growth conditions of our bl