Team:UCLA/Notebook/Honeybee Silk/19 July 2015

Lots of Colony PCR's

  • Ran Colony PCR's of both silk in T7 promoter biobrick, as well as silk in pet vector in BL21 cells.
  • The results from both looked weird, but there was at least a faint band at the right size.
  • We inoculated the 8 colonies from the t7 biobrick and also 6 colonies from the silk in bl21. (chlor and kan resistance respectively.)
  • Put tubes in at 7 pm 7/19 37 C