Team:UCLA/Notebook/Honeybee Silk/21 May 2015

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Inoculation of 5/19 Transformation

• I picked four colonies from the 1:1 kanamycin plate and suspended each in 100uL ddH2O
• Each sample was inoculated in 3 different culture tubes with 5mL LB broth and 5uL 1000X kanamycin and was incubated at 37C.