Team:UCLA/Notebook/Honeybee Silk/27 April 2015

iGEM UCLA




4/27 visualization and purification of honeybee gblock PCR

We visualized the gradient pcr from 4/26 on a 1% agarose gel. See image below:

  • The PCR for both constructs (silk coding region) and (regulatory elements + silk coding region) worked relatively well across all gradient temperatures.
  • We next scaled up the PCR reaction to 50 ul for each construct and gel extracted the product
  • From left to right, just silk w/ bb prefix and suffix w/ increasing annealing temp. , ladder, followed by silk+ promoter at increasing temp (see 4/26 for pcr conditions)