Team:UCLA/Notebook/Honeybee Silk/4 September 2015
SDS PAGE
- Running SDS PAGE in order to assess different lysis protocols
- The gel is running weird. The reason may be that the new gels we received from MArk Arbing require a special running buffer, and I used the Glycine running buffer that we have.
- Lanes
| Lane | Sample |
|---|---|
| 1 | BSA positive control |
| 2 | Negative control (water) |
| 3 | Philip's Lysis Protocol |
| 4 | Philip Lysis Full Cell Lysate (FCL) |
| 5 | Fasih's Lysis |
| 6 | Fasih' FCL |
| 7 | Bio rad dual color ladder |
| 8 | Fasigh's lysis (no iptg) |
| 9 | 1X concentrated silk |
| 10 | 3X Concentrated silk |
| 11 | Silk concentration flow through |
| 14 | Bug buster lysis |