Team:UCLA/Notebook/Honeybee Silk/5 August 2015

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8/5

• Analyzing dry film test.
  • Weight of 100 ul SDS solution after drying = 3.3mg
  • 5/26 Silk = 3.5 mg (subtracting the 3.3 mg of SDS = 0.2 mg
  • 7/29 silk = 20.5 mg or 17.2 mg after controlling for SDS.
• This corresponds to 0.2 weight by volume percent for the 5/26 Silk and 4 mg total for the 250 ml culture.
• Corresponds to a 17.2 w/v% for the 7/29 silk and 129 mg total for the 100 ml culture
• Once again, keep in mind that this is not necessarily all honeybee silk, and could be cellular debris and other contaminating proteins.

Starting 300ml Culture of Honeybee Silk

• 300 ml LB and 300 ul KAN, with one colony.
• Inoculated at noon
• At 4PM the OD was 0.06
• At 5:40 PM the OD was 0.56
  • At that point added 150 ul of 1M IPTG (0.5mM)
• Will incubate overnight.
• After overnight incubation, the OD was 2.42