Team:UCLA/Notebook/Interlab Study/13 August 2015

• HOME
• TEAM
• PROJECTS
  • Silk Genetics
  • Functionalization
  • Materials Processing
  • Protein Cages
• PARTS
  • Team Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
• NOTEBOOKS
  • Spider Silk Genetics
  • Honeybee Silk
  • Recombinant Silk Functionalization
  • Materials Processing
  • Protein Cages
  • UCLA Measurement Interlab Study
• MEETING NOTES
  • General Meetings
  • Coordinator Meetings
  • Advisor Meetings
• ATTRIBUTIONS
• COLLABORATIONS
• HUMAN PRACTICES
• SAFETY
• MEASUREMENT STUDY

Primer Arrival

Primers have been synthesized. Refer herefor complete data analysis of the primers.

Primers have been resuspended in Qiagen EB, briefly vortexed, and stored in -20 degrees Celsius. Forward primer suspended in 298 uL of EB (29.8 nmole pellet), and reverse primer suspended in 210 uL of EB (21 nmole pellet), for a final concentration of 100 uM in each primer stock.

For stock dilutions to working PCR aliquots, dilute both primers to 10uM. To get a 200 µL 10 µM solution, mix 20 µL of 100 µM oligo stock with 180 µL of nuclease free water. Briefly vortex, and place in -20 degrees Celsius.