Team:UCLA/Notebook/Materials/19 May 2015

• HOME
• TEAM
• PROJECTS
  • Silk Genetics
  • Functionalization
  • Materials Processing
  • Protein Cages
• PARTS
  • Team Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
• NOTEBOOKS
  • Spider Silk Genetics
  • Honeybee Silk
  • Recombinant Expression
  • Materials Processing
  • Protein Cages
• ATTRIBUTIONS
• COLLABORATIONS
• HUMAN PRACTICES
• SAFETY
• MODELING
• MEASUREMENT
• SOFTWARE
• ENTREPRENEURSHIP

Carter's part of today's notebook

• Carter's run of the wet spin
  • Procedure
    • Pipette 2ml 20% wt/vol cellulose acetate into syringe reservoir while plugging the narrow opening
    • Insert plunger, Invert and depress plunger until cellulose acetate is almost through the narrow opening
    • Attach 27.5 gauge needle
    • Attach syringe to syringe pump, run into ethanol at 16 microliters/min
  • Observations
    • Thick fibers, possibly just coils,very blue, wait for ethanol to rid the acetate

Jared's part of today's notebook

Dialysis of Silk in Lithium Bromide

• Cut 15 cm of dialysis tubing. Folded up bottom and clipped.
• Filled tubing with 20-25 mL of the silk/LiBr solution.
• Folded and clipped other end of tube and placed into 1L of ultrapure water.
• Silk was heavy enough to weigh down dialysis clips, so a floating thing was used to keep the tubing up.

Wet Spinning Extrusion Practice

• Placed 2mL of 20% w/v cellulose acetate in a syringe.
• Attached syringe to syringe pump. Attached 27.5 gauge needle.
• Positioned needle such that it descended vertically into a 70% ethanol solution.
• Ran the syringe pump at a rate of 6 uL/min.
• Fiber formation was not observed while the pump was running.
• While the pump was off, fiber formation was observed. Occasionally, the fiber was extruded in a coiled structure.

Michael's part of today's notebook

• Dialysis of 25 mL of silk
  • IMPORTANT NOTE: Since we're going over the volume recommended in the Kaplan paper, we'll likely need more water changes. Additionally, since we're using smaller pores than recommended in the Kaplan paper, we might need longer dialysis times in between changes.
  • start: 3:20 pm
  • 1st change: 4:20 pm
  • 2nd change: 3:50 pm, May 20
  • 3rd change: 2:00 pm, May 21
  • 4th change: 3:00 pm, May 24
  • 5th change: 4:00 pm, May 26
  • 6th change: 1:00 pm, May 27
  • 7th change: 5:00 pm, May 28

• Plan for the next few days:
  • we'll be concentrating and spinning silk.
    • Fixed factors:
      • PEEK tubing, 10 mm in length
      • stretch fibers by hand, 5x
      • Dope concentration of 15%. Aqueous.
    • Variable: coagulation dope
      • Ammonium sulfate
      • isopropanol
      • ethanol

• • Wednesday: start PEG dialysis at 4:00 PM
  • Thursday: stop PEG dialysis at 2:00 PM (22 hours)
    • aliquot 100 uL and place at 60 C to determine concentration
    • Dilute down to 15%
    • start spinning