Team:UCLA/Notebook/Materials/Lyophilizer

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Using the Seidlits lab's lyophilizer

• Using the lyophilizer
  • Press "auto" to start the vacuum and refrigeration
  • wait for the pressure and temperature to drop all the way down.
  • place frozen sample in a chamber, and place the lid on. Make sure that the lid is fully secure.
  • If there are already samples lyophilizing, be sure to close their valves before adding your chamber.
    • valves are open when the indentation is facing the tube leading to the chamber
  • push your chamber into a stem, then open the valve
  • then open the valves of the other chambers
  • If the alarm light is flashing, alert Alireza
  • To remove your sample, you only have to close the valve for your other chamber. You don't need to close the other chamber valves.
  • If you are the last one to use the lyophilizer, press "auto" again to turn off the machine
    • NOTE: Never turn off the side switch!
  • Then open the central chamber and take it off
  • use a large kimwipe to wipe off the water that accumulated inside the machine