Team:UCLA/Notebook/Protein Cages/20 August 2015
Phillip's notes:
Introduction: Today, DLS will be done on the purified PCquadY from last week. The elutions will be pooled before bringing it to Kevin.
Procedure: The first two elutions from the experiment visibly contained a white precipitate, which is likely the protein cage. These tubes were pooled and centrifuged. The pellet was re-suspended in 150uL of triton lysis buffer.
Results: The supernatant sample and the re-suspended pellet were both ran. Both did not provide a baseline reading.
Conclusion: Since aggregation seems to be a problem, the DLS may have to be ran soon after purification.