Team:UCLA/Notebook/Recombinant Expression/18 May 2015

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• Growing starter culture from grown Tamura insert pSB1A3 vector in NEB E. coli DH5alpha cells.
  • Inoculated 10 mL of Difco LB broth with 100ug*ul-1 concentration Carbenicillin with marked transformed colony.

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Transformed colonies from Tamura insert in pSB1A3 backbone. Grown using NEB E. coli K12 DH5alpha strains.

Julian's Work

• Last week I plated one of my glycerol stocks which had the Tamura construct This was verified via cPCR
  • did streak plating on a LB+Chlor plate
  • grew up at 37C overnight, colonies looked good