Team:UCLA/Notebook/Spider Silk Genetics/18 August 2015
iGEM UCLA
8/18/2015
Miniprep and Sequencing for M1-12(1C3)
- Miniprep four samples using Qiagen kit.
| M1-12(1C3)
|
Concentration (ng/uL)
|
A 260/280
|
| 1
|
261.78
|
1.83
|
| 2
|
295.50
|
1.80
|
| 3
|
311.02
|
1.81
|
| 4
|
272.50
|
1.84
|
- Sent samples for sequencinig.
Gel Purification
- Used zymo kit to purify BsaI digested M1-AB, BC, CA.
- yields around the 30 ng/uL mark.
Glycerol Stock Reconstitution
- Streaked out on individual plates M2-AB, BC, CA
- Streaked old and new glycerol stocks.
