Team:UCLA/Notebook/Spider Silk Genetics/23 July 2015

iGEM UCLA




7/23/2015

Sequencing Results for M2-9(T7)

  • All three samples have correct sequencing based on VF and VR.
  • Choose sample 2 as the one to give to the Protein Expression team.

Miniprep and Sequencing

  • Miniprep (Zymo) for M1-AB,BC,CA and M2-12(1C3).
    • Three samples each.
  • All samples were typical miniprep quality DNA.
    • Concentration was approx. 500 ng/uL
    • A 260/280 was between 1.82 and 1.89 for all samples.

ICA for M2-15

  • Olivia performed ICA for M2-15.
  • Used smaller wash volumes, and 4 minute ligation time.
  • Eluted in 0.01% Tween-20, but not amplified up.