Team:UCLA/Notebook/Spider Silk Genetics/24 June 2015

iGEM UCLA




6/24/2015

  • Cast 1.5% gel for visualize yesterday's ICA test results.
  • Used 2 uL 100 bp ladder.
Fig. 1 Post-elution primer amplification of Magnabind beads with the 3-mer construct consisting of Initiator-AB-BC-CA-Terminator. The expected product is ~400 bp long. No band is present at 400 bp.
  • The smear at 100 bp likely corresponds to primer dimers.
  • Perhaps we should conduct one more test with more controls.
  • This might be a bead problem, where the beads are not binding to the DNA.
  • Tomorrow, test binding entire IABCT construct onto beads, then amplifying.