Team:UCLA/Notebook/Spider Silk Genetics/26 August 2015

iGEM UCLA




8/26/2015

Transformation Results

  • Only 2 colonies present on the M1/2[1:2]-12(1C3) plate.
  • Possibly due to DNA loss during PCR purification.

Miniprep and Sequencing

  • Used Qiagen to miniprep 5 mL culture samples M1-12(1C3), M1/2[1:1]-12(1C3)/(T7), M1/2[2:1]-12(1C3)/(T7).
  • Sent to Genewiz for sequencing.
    • Used VF and VR primers for each.

Liquid Culture

  • Picked the two colonies into 5 mL LB + chlor.