Team:UCLA/Notebook/Spider Silk Genetics/31 July 2015
7/31/2015
Miniprep for M2-12(T7), M2-15(1C3)
- Used Qiagen Kit.
| Concentration | A 260/280 | |
|---|---|---|
| M2-12(T7) | 1110.94 | 1.88 |
| 1183.36 | 1.81 | |
| 873.87 | 1.88 | |
| M2-15(1C3) | 616.03 | 1.87 |
| 543.55 | 1.86 | |
| 181.27 | 1.81 |
- Sent genewiz for sequencing.
- M2-12(T7):
- Sequence with VF, VR.
- M2-15(1C3)
- Sequence with VF, M2-Seq-For, VR
- M2-12(T7):
BsaI Digestion of M1-AB, BC, CA
- Digest 2x 50 uL for each containing, 5 ug of plasmid in each, digest with 4 uL of BsaI.
- Digest for 2 hrs at 50 C, heat kill 65 C 20 min.
- Run results on 1.5% TAE t gel, with 2 uL of NEB 50 bp ladder.
- Excised the 102 bp band for gel extraction.