Team:UCLA/Notebook/Spider Silk Genetics/6 May 2015

iGEM UCLA




5/6/2015

5/6/2015


∙ Spun down yesterday’s cells in 50 mL tubes for 15 min @ 6000x g @ 4 C.

∙ Used Maxiprep (Qiagen) kit to prepare DNA. Eluted in 400 uL EB. Concentrations from each are listed below:

Amount (ng/uL)
2AB112
2BC205
2CA219

∙ The DNA amounts are low. We probably need to improve our technique.