Team:UCLA/Notebook/Spider Silk Genetics/8 September 2015

iGEM UCLA




9/8/2015

Miniprep and Sequencing for M1-15(T7)

  • Used qiagen kit to miniprep M1-15(T7) two colonies.
  • Sent to genewiz for sequenincing using VF, VR, and M1-SeqF2

Protein Purification

  • Made 5 L of LB in 5 separate bottles.
  • Streaked M1/2[1:2]-12(T7) and M1-12(T7)