Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/17 August 2015"
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+ | ='''Redo of Ligation of psb1c3+T7 promoter and Silk+GFP'''= | ||
+ | |||
+ | {| class="wikitable" style="text-align:center; width:400px; height:200px;" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Component | ||
+ | ! Volume (out of 20uL) | ||
+ | |- | ||
+ | ! T4 Ligase Reaction Buffer | ||
+ | | 2 ul | ||
+ | |- | ||
+ | ! Backbone (psb1c3+t7 promoter) | ||
+ | | 0.8uL (50 ng) | ||
+ | |- | ||
+ | ! Insert (silk + spyC) | ||
+ | | 1.5 ul (146 ng) | ||
+ | |- | ||
+ | ! H20 | ||
+ | | 14.7 ul | ||
+ | |- | ||
+ | ! Ligase | ||
+ | | 1uL | ||
+ | |} | ||
+ | *Program = 27C for 1 hour, heat inactivate for 10 minutes at 65C. | ||
+ | |||
=SDS PAGE: Determining Protein Quality= | =SDS PAGE: Determining Protein Quality= | ||
*Today we are testing multiple growth conditions of our bl | *Today we are testing multiple growth conditions of our bl |
Latest revision as of 21:50, 24 August 2015
Redo of Ligation of psb1c3+T7 promoter and Silk+GFP
Component | Volume (out of 20uL) |
---|---|
T4 Ligase Reaction Buffer | 2 ul |
Backbone (psb1c3+t7 promoter) | 0.8uL (50 ng) |
Insert (silk + spyC) | 1.5 ul (146 ng) |
H20 | 14.7 ul |
Ligase | 1uL |
- Program = 27C for 1 hour, heat inactivate for 10 minutes at 65C.
SDS PAGE: Determining Protein Quality
- Today we are testing multiple growth conditions of our bl