Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk"

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<summary>April 26 - May 2</summary>
 
<summary>April 26 - May 2</summary>
 
<details>
 
<details>
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<summary>4/26</summary>
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<summary>4/26 PCR off honey bee gene block</summary>
 +
 
 +
<ul>
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<li>See Benchling for the g block sequence and primer reference code https://benchling.com/s/p7bClpzQ/edit
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</li>
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<li>The goal of this is to clone 2  different constructs and prepare them as biobricks, one that is just the silk gene, and the other that as the promoter, rbs, etc.. required for protein expression.
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</li>
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<li>Restriction digest</li>
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<img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
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<li>Had a slice of pizza</li>
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</ul>
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</details>
 
</details>
  

Revision as of 05:30, 14 May 2015

iGEM UCLA




Honeybee Silk Notebook

April 26 - May 2
4/26 PCR off honey bee gene block
  • See Benchling for the g block sequence and primer reference code https://benchling.com/s/p7bClpzQ/edit
  • The goal of this is to clone 2 different constructs and prepare them as biobricks, one that is just the silk gene, and the other that as the promoter, rbs, etc.. required for protein expression.
  • Restriction digest
  • Had a slice of pizza
May 3 - May 8
5/4 Transformation of Silk and Prom + Silk
  • Using Sri’s chemically competent cells
  • Using ligation product from 4/30 (not purifying the ligation product as per Eric’s recommendation)
  • Restriction digest
  • Had a slice of pizza
Day 2
  • PCR
  • Ran gel
  • Restriction digest
    • Item Item Item Item
    Item Item Item Item
    Item Item Item Item
    1. item 1
    2. item 2
    3. item 3
    4. item 4
Day 3
  • PCR
  • Ran gel
  • Restriction digest
  • Had a slice of pizza