Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression"

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<h2>Recombinant Silk Functionalization</h2>
 
<h2>Recombinant Silk Functionalization</h2>
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<li>Ran gel</li>
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<li>Restriction digest</li>
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        <li> </li>
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<img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
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  </details>
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  <details>
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      <summary>mm/dd: Sample Entry</summary>
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      <p>Today we began cloning our GFP.</p>
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      <ul>
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        <li>PCR'd off template</li>
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        <li>Ran gel</li>
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        <li>Restriction digest</li>
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        <li>Ligated into backbone</li>
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      </ul>
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      <p>For our ligation, we made the following modifications:</p>
 +
      <ol>
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        <li>Tried it with newly bought ligase</li>
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        <li>Left reaction overnight instead of 2 hrs</li>
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        <li>Vector to insert ratio was 1:5 instead of 1:3</li>
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      </ol>
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      <p>PCR Reaction:</p>
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      <table style="width: 50%; text-align: center">
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        <tr>
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            <td><b>Component</b></td>
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            <td><b>Volume</b></td>
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        </tr>
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        <tr>
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            <td><b>5X Q5 Reaction Buffer</b></td>
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            <td>5</td>
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        </tr>
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            <td><b>10 mM dNTPs</b></td>
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            <td>0.5</td>
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        </tr>
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            <td><b>10 uM Forward (primer 3/7)</b></td>
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            <td>1.25</td>
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        </tr>
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        <tr>
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            <td><b>10 uM Reverse (primer 8)</b></td>
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            <td>1.25</td>
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        </tr>
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        <tr>
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            <td><b>Template (diluted to 1ng/uL)</b></td>
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            <td>0.5</td>
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        </tr>
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        <tr>
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            <td><b>Q5 High Fidelity DNA Polymerase</b></td>
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            <td>0.25</td>
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        </tr>
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        <tr>
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            <td><b>Nuclease Free Water</b></td>
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            <td>16.25</td>
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        </tr>
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      </table>
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      <br/>
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      <p>Gel: Lot of bands, all at correct sizes</p>
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      <img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
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  </details>
 
</details>
 
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Revision as of 21:39, 15 May 2015

iGEM UCLA




Recombinant Silk Functionalization

mm/dd - mm/dd (most recent weeks on top)
mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.


Unordered lists for bullet points


For images, upload image onto wiki server. Then replace placeholder image url with your image's url.



Ordered lists for numbers


For tables, "tr" stands for "table row", and "td" means "table column."

Row 1, Column 1 Row 1, Column 2 Row 1, Column 3 Row 1, Column 4
Row 2, Column 1 Row 2, Column 2 Row 2, Column 3 Row 2, Column 4
Row 1, Column 1 Row 2, Column 2 Row 3, Column 3 Row 4, Column 4

For different types of text styling... bold, italics, underlined. For headers...

Headers

Smaller Header

You get the point

Feel free to google any other styling.


Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

  • PCR'd off template
  • Ran gel
  • Restriction digest
  • Ligated into backbone

For our ligation, we made the following modifications:

  1. Tried it with newly bought ligase
  2. Left reaction overnight instead of 2 hrs
  3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

Component Volume
5X Q5 Reaction Buffer 5
10 mM dNTPs 0.5
10 uM Forward (primer 3/7) 1.25
10 uM Reverse (primer 8) 1.25
Template (diluted to 1ng/uL) 0.5
Q5 High Fidelity DNA Polymerase 0.25
Nuclease Free Water 16.25

Gel: Lot of bands, all at correct sizes