Difference between revisions of "Team:UCLA/Notebook"

 
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<details>
 
<details>
−
<summary>mm/dd - mm/dd (most recent weeks on top)</summary>
+
  <summary>mm/dd - mm/dd (most recent weeks on top)</summary>
−
<details>
+
  <details>
−
<summary>mm/dd (most recent days on top): Entry title/short description</summary>
+
      <summary>mm/dd: Entry title/short description (most recent days on top)</summary>
−
<p>Plain text for descriptions, etc.</p>
+
      <p>Plain text for descriptions, etc.</p>
−
<br/>
+
      <br/>
−
<p>Unordered lists for bullet points</p>
+
      <p>Unordered lists for bullet points</p>
−
<ul>
+
      <ul>
−
<li>Bulletpoint 1</li>
+
        <li> </li>
−
<li>Bulletpoint 2</li>
+
        <li></li>
−
<li>Bulletpoint 3</li>
+
        <li></li>
−
</ul>
+
      </ul>
−
<br/>
+
      <br/>
−
<p>For images, upload image onto wiki server. Then replace placeholder image url with your image's url.</p>
+
      <p>For images, upload image onto wiki server. Then replace placeholder image url with your image's url.</p>
−
<img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
+
      <img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
−
<br/>
+
      <br/>
−
<br/>
+
      <br/>
−
<p>Ordered lists for numbers</p>
+
      <p>Ordered lists for numbers</p>
−
<ol>
+
      <ol>
−
<li>Number 1</li>
+
        <li></li>
−
<li>Number 2</li>
+
        <li></li>
−
<li>Number 3</li>
+
        <li></li>
−
</ol>
+
      </ol>
−
<br/>
+
      <br/>
−
<p>For tables, "tr" stands for "table row", and "td" means "table column."</p>
+
      <p>For tables, "tr" stands for "table row", and "td" means "table column."</p>
−
<table style="width: 100%; text-align: center">
+
      <table style="width: 50%; text-align: center">
−
<tr>
+
        <tr>
−
<td><b>Row 1, Column 1</b></td>
+
            <td><b>Row 1, Column 1</b></td>
−
<td><b>Row 1, Column 2</b></td>
+
            <td><b>Row 1, Column 2</b></td>
−
<td><b>Row 1, Column 3</b></td>
+
            <td><b>Row 1, Column 3</b></td>
−
<td><b>Row 1, Column 4</b></td>
+
            <td><b>Row 1, Column 4</b></td>
−
</tr>
+
        </tr>
−
<tr>
+
        <tr>
−
<td><b>Row 2, Column 1</b></td>
+
            <td><b>Row 2, Column 1</b></td>
−
<td>Row 2, Column 2</td>
+
            <td>Row 2, Column 2</td>
−
<td>Row 2, Column 3</td>
+
            <td>Row 2, Column 3</td>
−
<td>Row 2, Column 4</td>
+
            <td>Row 2, Column 4</td>
−
</tr>
+
        </tr>
−
<tr>
+
        <tr>
−
<td><b>Row 1, Column 1</b></td>
+
            <td><b>Row 1, Column 1</b></td>
−
<td>Row 2, Column 2</td>
+
            <td>Row 2, Column 2</td>
−
<td>Row 3, Column 3</td>
+
            <td>Row 3, Column 3</td>
−
<td>Row 4, Column 4</td>
+
            <td>Row 4, Column 4</td>
−
</tr>
+
        </tr>
−
</table>
+
      </table>
−
<br/>
+
      <br/>
−
<p>
+
      <p>For different types of text styling... <b>bold</b>, <i>italics</i>, <u>underlined</u>. For headers... </p>
−
For different types of text styling... <b>bold</b>, <i>italics</i>, <u>underlined</u>. For headers... </p>
+
      <h1>Headers</h1>
−
<h1>Headers</h1>
+
      <h2>Smaller Header</h2>
−
<h2>Smaller Header</h2>
+
      <h3>You get the point</h3>
−
<h3>You get the point</h3>
+
      <p>Feel free to google any other styling.</p>
−
<p>Feel free to google any other styling.</p>
+
      <br/>
−
<br/>
+
      <p>Keep all your html within the detail tags for the day/week you're putting your entry for.</p>
−
<p>Keep all your html within the detail tags for the day/week you're putting your entry for.</p>
+
      <p>To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html</p>
−
</details>
+
  </details>
−
<details>
+
  <details>
−
<summary>Day 2</summary>
+
      <summary>mm/dd: Sample Entry</summary>
−
<ul>
+
      <p>Today we began cloning our GFP.</p>
−
<li>PCR</li>
+
      <ul>
−
<li>Ran gel</li>
+
        <li>PCR'd off template</li>
−
<li>Restriction digest</li>
+
        <li>Ran gel</li>
−
<table style="width: 100%">
+
        <li>Restriction digest</li>
−
<tr>
+
        <li>Ligated into backbone</li>
−
<td><b>Item</b></td>
+
      </ul>
−
<td><b>Item</b></td>
+
      <p>For our ligation, we made the following modifications:</p>
−
<td><b>Item</b></td>
+
      <ol>
−
<td><b>Item</b></td>
+
        <li>Tried it with newly bought ligase</li>
−
</tr>
+
        <li>Left reaction overnight instead of 2 hrs</li>
−
<tr>
+
        <li>Vector to insert ratio was 1:5 instead of 1:3</li>
−
<td><b>Item</b></td>
+
      </ol>
−
<td>Item</td>
+
      <p>PCR Reaction:</p>
−
<td>Item</td>
+
      <table style="width: 50%; text-align: center">
−
<td>Item</td>
+
        <tr>
−
</tr>
+
            <td><b>Component</b></td>
−
<tr>
+
            <td><b>Volume</b></td>
−
<td><b>Item</b></td>
+
        </tr>
−
<td>Item</td>
+
        <tr>
−
<td>Item</td>
+
            <td><b>5X Q5 Reaction Buffer</b></td>
−
<td>Item</td>
+
            <td>5</td>
−
</tr>
+
        </tr>
−
</table>
+
        <tr>
−
</ul>
+
            <td><b>10 mM dNTPs</b></td>
−
</details>
+
            <td>0.5</td>
−
<details>
+
        </tr>
−
<summary>Day 3</summary>
+
        <tr>
−
<ul>
+
            <td><b>10 uM Forward (primer 3/7)</b></td>
−
<li>PCR</li>
+
            <td>1.25</td>
−
<li>Ran gel</li>
+
        </tr>
−
<li>Restriction digest</li>
+
        <tr>
−
<li>Had a slice of pizza</li>
+
            <td><b>10 uM Reverse (primer 8)</b></td>
−
</ul>
+
            <td>1.25</td>
−
</details>
+
        </tr>
 +
        <tr>
 +
            <td><b>Template (diluted to 1ng/uL)</b></td>
 +
            <td>0.5</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Q5 High Fidelity DNA Polymerase</b></td>
 +
            <td>0.25</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Nuclease Free Water</b></td>
 +
            <td>16.25</td>
 +
        </tr>
 +
      </table>
 +
      <br/>
 +
      <p>Gel: Lot of bands, all at correct sizes</p>
 +
      <img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
 +
  </details>
 
</details>
 
</details>
  
  
 
<details>
 
<details>
−
<summary>Week 2</summary>
+
  <summary>mm/dd - mm/dd (most recent weeks on top)</summary>
−
<details>
+
  <details>
−
<summary>Day 1</summary>
+
      <summary>mm/dd: Entry title/short description (most recent days on top)</summary>
−
<ul>
+
      <p>Plain text for descriptions, etc.</p>
−
<li>PCR</li>
+
      <br/>
−
<li>Ran gel</li>
+
      <p>Unordered lists for bullet points</p>
−
<li>Restriction digest</li>
+
      <ul>
−
<li>Had a slice of pizza</li>
+
        <li>Bulletpoint 1</li>
−
</ul>
+
        <li>Bulletpoint 2</li>
−
</details>
+
        <li>Bulletpoint 3</li>
−
<details>
+
      </ul>
−
<summary>Day 2</summary>
+
      <br/>
−
<ul>
+
      <p>For images, upload image onto wiki server. Then replace placeholder image url with your image's url.</p>
−
<li>PCR</li>
+
      <img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
−
<li>Ran gel</li>
+
      <br/>
−
<li>Restriction digest</li>
+
      <br/>
−
<li>Had a slice of pizza</li>
+
      <p>Ordered lists for numbers</p>
−
</ul>
+
      <ol>
−
</details>
+
        <li>Number 1</li>
−
 
+
        <li>Number 2</li>
 +
        <li>Number 3</li>
 +
      </ol>
 +
      <br/>
 +
      <p>For tables, "tr" stands for "table row", and "td" means "table column."</p>
 +
      <table style="width: 50%; text-align: center">
 +
        <tr>
 +
            <td><b>Row 1, Column 1</b></td>
 +
            <td><b>Row 1, Column 2</b></td>
 +
            <td><b>Row 1, Column 3</b></td>
 +
            <td><b>Row 1, Column 4</b></td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Row 2, Column 1</b></td>
 +
            <td>Row 2, Column 2</td>
 +
            <td>Row 2, Column 3</td>
 +
            <td>Row 2, Column 4</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Row 1, Column 1</b></td>
 +
            <td>Row 2, Column 2</td>
 +
            <td>Row 3, Column 3</td>
 +
            <td>Row 4, Column 4</td>
 +
        </tr>
 +
      </table>
 +
      <br/>
 +
      <p>For different types of text styling... <b>bold</b>, <i>italics</i>, <u>underlined</u>. For headers... </p>
 +
      <h1>Headers</h1>
 +
      <h2>Smaller Header</h2>
 +
      <h3>You get the point</h3>
 +
      <p>Feel free to google any other styling.</p>
 +
      <br/>
 +
      <p>Keep all your html within the detail tags for the day/week you're putting your entry for.</p>
 +
      <p>To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html</p>
 +
  </details>
 +
  <details>
 +
      <summary>mm/dd: Sample Entry</summary>
 +
      <p>Today we began cloning our GFP.</p>
 +
      <ul>
 +
        <li>PCR'd off template</li>
 +
        <li>Ran gel</li>
 +
        <li>Restriction digest</li>
 +
        <li>Ligated into backbone</li>
 +
      </ul>
 +
      <p>For our ligation, we made the following modifications:</p>
 +
      <ol>
 +
        <li>Tried it with newly bought ligase</li>
 +
        <li>Left reaction overnight instead of 2 hrs</li>
 +
        <li>Vector to insert ratio was 1:5 instead of 1:3</li>
 +
      </ol>
 +
      <p>PCR Reaction:</p>
 +
      <table style="width: 50%; text-align: center">
 +
        <tr>
 +
            <td><b>Component</b></td>
 +
            <td><b>Volume</b></td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>5X Q5 Reaction Buffer</b></td>
 +
            <td>5</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>10 mM dNTPs</b></td>
 +
            <td>0.5</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>10 uM Forward (primer 3/7)</b></td>
 +
            <td>1.25</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>10 uM Reverse (primer 8)</b></td>
 +
            <td>1.25</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Template (diluted to 1ng/uL)</b></td>
 +
            <td>0.5</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Q5 High Fidelity DNA Polymerase</b></td>
 +
            <td>0.25</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Nuclease Free Water</b></td>
 +
            <td>16.25</td>
 +
        </tr>
 +
      </table>
 +
      <br/>
 +
      <p>Gel: Lot of bands, all at correct sizes</p>
 +
      <img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
 +
  </details>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Week 1</summary>
+
  <summary>mm/dd - mm/dd (most recent weeks on top)</summary>
−
<details>
+
  <details>
−
<summary>Day 1</summary>
+
      <summary>mm/dd: Entry title/short description (most recent days on top)</summary>
−
<ul>
+
      <p>Plain text for descriptions, etc.</p>
−
<li>PCR</li>
+
      <br/>
−
<li>Ran gel</li>
+
      <p>Unordered lists for bullet points</p>
−
<li>Restriction digest</li>
+
      <ul>
−
<li>Had a slice of pizza</li>
+
        <li>Bulletpoint 1</li>
−
</ul>
+
        <li>Bulletpoint 2</li>
−
</details>
+
        <li>Bulletpoint 3</li>
 +
      </ul>
 +
      <br/>
 +
      <p>For images, upload image onto wiki server. Then replace placeholder image url with your image's url.</p>
 +
      <img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
 +
      <br/>
 +
      <br/>
 +
      <p>Ordered lists for numbers</p>
 +
      <ol>
 +
        <li>Number 1</li>
 +
        <li>Number 2</li>
 +
        <li>Number 3</li>
 +
      </ol>
 +
      <br/>
 +
      <p>For tables, "tr" stands for "table row", and "td" means "table column."</p>
 +
      <table style="width: 50%; text-align: center">
 +
        <tr>
 +
            <td><b>Row 1, Column 1</b></td>
 +
            <td><b>Row 1, Column 2</b></td>
 +
            <td><b>Row 1, Column 3</b></td>
 +
            <td><b>Row 1, Column 4</b></td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Row 2, Column 1</b></td>
 +
            <td>Row 2, Column 2</td>
 +
            <td>Row 2, Column 3</td>
 +
            <td>Row 2, Column 4</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Row 1, Column 1</b></td>
 +
            <td>Row 2, Column 2</td>
 +
            <td>Row 3, Column 3</td>
 +
            <td>Row 4, Column 4</td>
 +
        </tr>
 +
      </table>
 +
      <br/>
 +
      <p>For different types of text styling... <b>bold</b>, <i>italics</i>, <u>underlined</u>. For headers... </p>
 +
      <h1>Headers</h1>
 +
      <h2>Smaller Header</h2>
 +
      <h3>You get the point</h3>
 +
      <p>Feel free to google any other styling.</p>
 +
      <br/>
 +
      <p>Keep all your html within the detail tags for the day/week you're putting your entry for.</p>
 +
      <p>To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html</p>
 +
  </details>
 +
  <details>
 +
      <summary>mm/dd: Sample Entry</summary>
 +
      <p>Today we began cloning our GFP.</p>
 +
      <ul>
 +
        <li>PCR'd off template</li>
 +
        <li>Ran gel</li>
 +
        <li>Restriction digest</li>
 +
        <li>Ligated into backbone</li>
 +
      </ul>
 +
      <p>For our ligation, we made the following modifications:</p>
 +
      <ol>
 +
        <li>Tried it with newly bought ligase</li>
 +
        <li>Left reaction overnight instead of 2 hrs</li>
 +
        <li>Vector to insert ratio was 1:5 instead of 1:3</li>
 +
      </ol>
 +
      <p>PCR Reaction:</p>
 +
      <table style="width: 50%; text-align: center">
 +
        <tr>
 +
            <td><b>Component</b></td>
 +
            <td><b>Volume</b></td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>5X Q5 Reaction Buffer</b></td>
 +
            <td>5</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>10 mM dNTPs</b></td>
 +
            <td>0.5</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>10 uM Forward (primer 3/7)</b></td>
 +
            <td>1.25</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>10 uM Reverse (primer 8)</b></td>
 +
            <td>1.25</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Template (diluted to 1ng/uL)</b></td>
 +
            <td>0.5</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Q5 High Fidelity DNA Polymerase</b></td>
 +
            <td>0.25</td>
 +
        </tr>
 +
        <tr>
 +
            <td><b>Nuclease Free Water</b></td>
 +
            <td>16.25</td>
 +
        </tr>
 +
      </table>
 +
      <br/>
 +
      <p>Gel: Lot of bands, all at correct sizes</p>
 +
      <img width="300px" src="https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg"/>
 +
  </details>
 
</details>
 
</details>
 +
 +
 +
  
 
<br></br>
 
<br></br>
Line 141: Line 347:
 
<br></br>
 
<br></br>
  
 +
 +
 +
<!--
 
<p> Document the dates you worked on your project.</p>
 
<p> Document the dates you worked on your project.</p>
  
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{{#calendar: year=2015 | month=july | title = Team:UCLA/Notebook/org | query=preload=Template:UCLA}}
 
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Latest revision as of 23:38, 20 May 2015

iGEM UCLA




Notebook Template

mm/dd - mm/dd (most recent weeks on top)
mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.


Unordered lists for bullet points


For images, upload image onto wiki server. Then replace placeholder image url with your image's url.



Ordered lists for numbers


For tables, "tr" stands for "table row", and "td" means "table column."

Row 1, Column 1 Row 1, Column 2 Row 1, Column 3 Row 1, Column 4
Row 2, Column 1 Row 2, Column 2 Row 2, Column 3 Row 2, Column 4
Row 1, Column 1 Row 2, Column 2 Row 3, Column 3 Row 4, Column 4

For different types of text styling... bold, italics, underlined. For headers...

Headers

Smaller Header

You get the point

Feel free to google any other styling.


Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

  • PCR'd off template
  • Ran gel
  • Restriction digest
  • Ligated into backbone

For our ligation, we made the following modifications:

  1. Tried it with newly bought ligase
  2. Left reaction overnight instead of 2 hrs
  3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

Component Volume
5X Q5 Reaction Buffer 5
10 mM dNTPs 0.5
10 uM Forward (primer 3/7) 1.25
10 uM Reverse (primer 8) 1.25
Template (diluted to 1ng/uL) 0.5
Q5 High Fidelity DNA Polymerase 0.25
Nuclease Free Water 16.25

Gel: Lot of bands, all at correct sizes

mm/dd - mm/dd (most recent weeks on top)
mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.


Unordered lists for bullet points

  • Bulletpoint 1
  • Bulletpoint 2
  • Bulletpoint 3

For images, upload image onto wiki server. Then replace placeholder image url with your image's url.



Ordered lists for numbers

  1. Number 1
  2. Number 2
  3. Number 3

For tables, "tr" stands for "table row", and "td" means "table column."

Row 1, Column 1 Row 1, Column 2 Row 1, Column 3 Row 1, Column 4
Row 2, Column 1 Row 2, Column 2 Row 2, Column 3 Row 2, Column 4
Row 1, Column 1 Row 2, Column 2 Row 3, Column 3 Row 4, Column 4

For different types of text styling... bold, italics, underlined. For headers...

Headers

Smaller Header

You get the point

Feel free to google any other styling.


Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

  • PCR'd off template
  • Ran gel
  • Restriction digest
  • Ligated into backbone

For our ligation, we made the following modifications:

  1. Tried it with newly bought ligase
  2. Left reaction overnight instead of 2 hrs
  3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

Component Volume
5X Q5 Reaction Buffer 5
10 mM dNTPs 0.5
10 uM Forward (primer 3/7) 1.25
10 uM Reverse (primer 8) 1.25
Template (diluted to 1ng/uL) 0.5
Q5 High Fidelity DNA Polymerase 0.25
Nuclease Free Water 16.25

Gel: Lot of bands, all at correct sizes

mm/dd - mm/dd (most recent weeks on top)
mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.


Unordered lists for bullet points

  • Bulletpoint 1
  • Bulletpoint 2
  • Bulletpoint 3

For images, upload image onto wiki server. Then replace placeholder image url with your image's url.



Ordered lists for numbers

  1. Number 1
  2. Number 2
  3. Number 3

For tables, "tr" stands for "table row", and "td" means "table column."

Row 1, Column 1 Row 1, Column 2 Row 1, Column 3 Row 1, Column 4
Row 2, Column 1 Row 2, Column 2 Row 2, Column 3 Row 2, Column 4
Row 1, Column 1 Row 2, Column 2 Row 3, Column 3 Row 4, Column 4

For different types of text styling... bold, italics, underlined. For headers...

Headers

Smaller Header

You get the point

Feel free to google any other styling.


Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

  • PCR'd off template
  • Ran gel
  • Restriction digest
  • Ligated into backbone

For our ligation, we made the following modifications:

  1. Tried it with newly bought ligase
  2. Left reaction overnight instead of 2 hrs
  3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

Component Volume
5X Q5 Reaction Buffer 5
10 mM dNTPs 0.5
10 uM Forward (primer 3/7) 1.25
10 uM Reverse (primer 8) 1.25
Template (diluted to 1ng/uL) 0.5
Q5 High Fidelity DNA Polymerase 0.25
Nuclease Free Water 16.25

Gel: Lot of bands, all at correct sizes