Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/18 May 2015"

 
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**Inoculated 10 mL of Difco LB broth with 100ug*ul-1 concentration Carbenicillin with marked transformed colony.
 
**Inoculated 10 mL of Difco LB broth with 100ug*ul-1 concentration Carbenicillin with marked transformed colony.
 
[[File:UCLA iGEM Tamura 05 Plate 5-18.jpg|200px|thumb|left|Transformed colonies from Tamura insert in pSB1A3 backbone.  Grown using NEB E. coli K12 DH5alpha strains.]]
 
[[File:UCLA iGEM Tamura 05 Plate 5-18.jpg|200px|thumb|left|Transformed colonies from Tamura insert in pSB1A3 backbone.  Grown using NEB E. coli K12 DH5alpha strains.]]
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=Julian's Work=
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*Last week I plated one of my glycerol stocks which had the Tamura construct This was verified via cPCR
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**did streak plating on a LB+Chlor plate
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**grew up at 37C overnight, colonies looked good

Latest revision as of 23:00, 22 May 2015


  • Growing starter culture from grown Tamura insert pSB1A3 vector in NEB E. coli DH5alpha cells.
    • Inoculated 10 mL of Difco LB broth with 100ug*ul-1 concentration Carbenicillin with marked transformed colony.
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Transformed colonies from Tamura insert in pSB1A3 backbone. Grown using NEB E. coli K12 DH5alpha strains.


Julian's Work

  • Last week I plated one of my glycerol stocks which had the Tamura construct This was verified via cPCR
    • did streak plating on a LB+Chlor plate
    • grew up at 37C overnight, colonies looked good