Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/16 May 2015"
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| + | =Plating Bacteria for Honeybee Silk Protein Expression= | ||
| + | I am plating these bacterial glycerol stocks in order to get colonies, which I will inoculate in order to express and purify our honeybee silk protein. | ||
*Plated cells on choramphenicol agar plates using glycerol stocks of [https://benchling.com/s/TxD8z7Kq/edit regulatory elements + silk#3 coding] | *Plated cells on choramphenicol agar plates using glycerol stocks of [https://benchling.com/s/TxD8z7Kq/edit regulatory elements + silk#3 coding] | ||
**Out of the three tubes of glycerol stocks, I used tube #1 on plate #1 and tube #2 on plate #2. | **Out of the three tubes of glycerol stocks, I used tube #1 on plate #1 and tube #2 on plate #2. | ||
**I spread the bacteria using a bacterial streaker instead of beads | **I spread the bacteria using a bacterial streaker instead of beads | ||
Latest revision as of 00:20, 23 May 2015
Plating Bacteria for Honeybee Silk Protein Expression
I am plating these bacterial glycerol stocks in order to get colonies, which I will inoculate in order to express and purify our honeybee silk protein.
- Plated cells on choramphenicol agar plates using glycerol stocks of regulatory elements + silk#3 coding
- Out of the three tubes of glycerol stocks, I used tube #1 on plate #1 and tube #2 on plate #2.
- I spread the bacteria using a bacterial streaker instead of beads