Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/16 May 2015"

 
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=Plating Bacteria for Honeybee Silk Protein Expression=
 
=Plating Bacteria for Honeybee Silk Protein Expression=

Latest revision as of 00:20, 23 May 2015

iGEM UCLA




Plating Bacteria for Honeybee Silk Protein Expression

I am plating these bacterial glycerol stocks in order to get colonies, which I will inoculate in order to express and purify our honeybee silk protein.

  • Plated cells on choramphenicol agar plates using glycerol stocks of regulatory elements + silk#3 coding
    • Out of the three tubes of glycerol stocks, I used tube #1 on plate #1 and tube #2 on plate #2.
    • I spread the bacteria using a bacterial streaker instead of beads