Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/26 August 2015"
Vinsonclam (Talk | contribs) (Created page with "{{Template:UCLA}}} =8/26/2015= ==Transformation Results== *Only 2 colonies present on the M1/2[1:2]-12(1C3) plate. *Possibly due to DNA loss during PCR purification. ==Minipre...") |
(No difference)
|
Revision as of 16:21, 31 August 2015
}
8/26/2015
Transformation Results
- Only 2 colonies present on the M1/2[1:2]-12(1C3) plate.
- Possibly due to DNA loss during PCR purification.
Miniprep and Sequencing
- Used Qiagen to miniprep 5 mL culture samples M1-12(1C3), M1/2[1:1]-12(1C3)/(T7), M1/2[2:1]-12(1C3)/(T7).
- Sent to Genewiz for sequencing.
- Used VF and VR primers for each.
Liquid Culture
- Picked the two colonies into 5 mL LB + chlor.