Difference between revisions of "Team:Vilnius-Lithuania/Labjournal"

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                        <h3 style="padding: 0px; margin: 0px;">Parts construction</h3>
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Part: <strong>P14.2</strong> (BBa_K1773006)
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Written by: <strong>IR</strong><br />
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Performed by: <strong>ŠT</strong>, <strong>IR</strong>, <strong>MB</strong>, <strong>IS</strong>.
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  <h3><strong>About</strong></h3>
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  <p>pLac+cI.<br />P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.</p>
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        <th>Date</th>
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        <th>Materials</th>
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        <th>Experiment</th>
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        <td>06 30</td>
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        <td>P10<br />P11</td>
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        <td>P10 and P11 transformation into DH5α.</td>
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        <td>07 01</td>
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        <td></td>
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        <td>Bacteria inoculation (4 minipreps).</td>
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        <td>07 09</td>
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        <td>P10<br />P11</td>
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        <td>
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Plasmids’ restriction (3 ug each).<br/>
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P10 with EcoRI and XBal (+FastAp).<br/>
 +
P11 with EcoRI and SpeI.<br/>
 +
Extraction from gel<br/>
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L10 and L11 are produced.<br/>
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Conc.: L11 – 2,4 ng/uL.
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  <h3><strong>Conclusions and Results:</strong></h3>
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  <p style="height: auto; margin: auto;">07 14 – all of the colonies are successful, except third colony.</p>
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Revision as of 22:06, 3 September 2015

About

pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.

Date Materials Experiment
06 30 P10
P11		 P10 and P11 transformation into DH5α.
07 01 Bacteria inoculation (4 minipreps).
07 09 P10
P11		 Plasmids’ restriction (3 ug each).
P10 with EcoRI and XBal (+FastAp).
P11 with EcoRI and SpeI.
Extraction from gel
L10 and L11 are produced.
Conc.: L11 – 2,4 ng/uL.

Conclusions and Results:

07 14 – all of the colonies are successful, except third colony.