Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/4 September 2015"

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Revision as of 02:06, 5 September 2015

iGEM UCLA




SDS PAGE

  • Running SDS PAGE in order to assess different lysis protocols
  • The gel is running weird. The reason may be that the new gels we received from MArk Arbing require a special running buffer, and I used the Glycine running buffer that we have.
  • Lanes
    • {| class="wikitable"

! Lane ! Sample |- | 1 | BSA positive control |- | 2 | Negative control (water) |- | 3 | Philip's Lysis Protocol |- | 4 | Philip Lysis Full Cell Lysate (FCL) |- | 5 | Fasih's Lysis |- | 6 | Fasih' FCL |- | 7 | Bio rad dual color ladder |- | 8 | Fasigh's lysis (no iptg) |- | 9 | 1X concentrated silk |- | 10 | 3X Concentrated silk |- | 11 | Silk concentration flow through |- | 14 | Bug buster lysis |}