Difference between revisions of "Team:Vilnius-Lithuania/Labjournal"
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<!-- /MethodBox --> | <!-- /MethodBox --> | ||
+ | <!-- MethodBox --> | ||
+ | <div class="panel panel-default" style="position: relative"> | ||
+ | <div class="panel-heading"> | ||
+ | <a class="accordion-toggle collapsed" data-toggle="collapse" data-parent="#accordion" href="#2"> | ||
+ | <div class="panel-title"> | ||
+ | <h3 style="padding: 0px; margin: 0px;">Parts construction</h3> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | Part: <strong>P14.2</strong> (BBa_K1773006) | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | Written by: <strong>IR</strong><br /> | ||
+ | Performed by: <strong>ŠT</strong>, <strong>IR</strong>, <strong>MB</strong>, <strong>IS</strong>. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div id="2" class="panel-collapse collapse"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-12"> | ||
+ | <h3><strong>About</strong></h3> | ||
+ | <p>pLac+cI.<br />P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row"> | ||
+ | <div class="col-md-12"> | ||
+ | <table class="table"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Date</th> | ||
+ | <th>Materials</th> | ||
+ | <th>Experiment</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>06 30</td> | ||
+ | <td>P10<br />P11</td> | ||
+ | <td>P10 and P11 transformation into DH5α.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>07 01</td> | ||
+ | <td></td> | ||
+ | <td>Bacteria inoculation (4 minipreps).</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>07 09</td> | ||
+ | <td>P10<br />P11</td> | ||
+ | <td> | ||
+ | Plasmids’ restriction (3 ug each).<br/> | ||
+ | P10 with EcoRI and XBal (+FastAp).<br/> | ||
+ | P11 with EcoRI and SpeI.<br/> | ||
+ | Extraction from gel<br/> | ||
+ | L10 and L11 are produced.<br/> | ||
+ | Conc.: L11 – 2,4 ng/uL. | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row"> | ||
+ | <div class="col-md-12"> | ||
+ | <h3><strong>Conclusions and Results:</strong></h3> | ||
+ | <p style="height: auto; margin: auto;">07 14 – all of the colonies are successful, except third colony.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- /MethodBox --> | ||
Revision as of 20:36, 7 September 2015
About
pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.
Date | Materials | Experiment |
---|---|---|
06 30 | P10 P11 |
P10 and P11 transformation into DH5α. |
07 01 | Bacteria inoculation (4 minipreps). | |
07 09 | P10 P11 |
Plasmids’ restriction (3 ug each). P10 with EcoRI and XBal (+FastAp). P11 with EcoRI and SpeI. Extraction from gel L10 and L11 are produced. Conc.: L11 – 2,4 ng/uL. |
Conclusions and Results:
07 14 – all of the colonies are successful, except third colony.
About
pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.
Date | Materials | Experiment |
---|---|---|
06 30 | P10 P11 |
P10 and P11 transformation into DH5α. |
07 01 | Bacteria inoculation (4 minipreps). | |
07 09 | P10 P11 |
Plasmids’ restriction (3 ug each). P10 with EcoRI and XBal (+FastAp). P11 with EcoRI and SpeI. Extraction from gel L10 and L11 are produced. Conc.: L11 – 2,4 ng/uL. |
Conclusions and Results:
07 14 – all of the colonies are successful, except third colony.