Difference between revisions of "Team:Vilnius-Lithuania/Labjournal"

Line 195: Line 195:
 
<!-- /MethodBox -->
 
<!-- /MethodBox -->
  
 +
<!-- MethodBox -->
 +
                <div class="panel panel-default" style="position: relative">
 +
                    <div class="panel-heading">
 +
                        <a class="accordion-toggle collapsed" data-toggle="collapse" data-parent="#accordion" href="#3">
 +
                        <div class="panel-title">
 +
                        <h3 style="padding: 0px; margin: 0px;">Parts construction</h3>
 +
<div class="row">
 +
  <div class="col-md-6">
 +
Part: <strong>P14.2</strong> (BBa_K1773006)
 +
  </div>
 +
  <div class="col-md-6">
 +
Written by: <strong>IR</strong><br />
 +
Performed by: <strong>ŠT</strong>, <strong>IR</strong>, <strong>MB</strong>, <strong>IS</strong>.
 +
  </div>
 +
</div>
 +
                        </div>
 +
                        </a>
 +
                    </div>
 +
                    <div id="3" class="panel-collapse collapse">
 +
                        <div class="panel-body">
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <h3><strong>About</strong></h3>
 +
  <p>pLac+cI.<br />P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.</p>
 +
  </div>
 +
</div>
 +
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <table class="table">
 +
    <thead>
 +
      <tr>
 +
        <th>Date</th>
 +
        <th>Materials</th>
 +
        <th>Experiment</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 +
      <tr>
 +
        <td>06 30</td>
 +
        <td>P10<br />P11</td>
 +
        <td>P10 and P11 transformation into DH5α.</td>
 +
      </tr>
 +
      <tr>
 +
        <td>07 01</td>
 +
        <td></td>
 +
        <td>Bacteria inoculation (4 minipreps).</td>
 +
      </tr>
 +
      <tr>
 +
        <td>07 09</td>
 +
        <td>P10<br />P11</td>
 +
        <td>
 +
Plasmids’ restriction (3 ug each).<br/>
 +
P10 with EcoRI and XBal (+FastAp).<br/>
 +
P11 with EcoRI and SpeI.<br/>
 +
Extraction from gel<br/>
 +
L10 and L11 are produced.<br/>
 +
Conc.: L11 – 2,4 ng/uL.
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 +
  </div>
 +
</div>
 +
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <h3><strong>Conclusions and Results:</strong></h3>
 +
  <p style="height: auto; margin: auto;">07 14 – all of the colonies are successful, except third colony.</p>
 +
  </div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
<!-- /MethodBox -->
 +
 +
<!-- MethodBox -->
 +
                <div class="panel panel-default" style="position: relative">
 +
                    <div class="panel-heading">
 +
                        <a class="accordion-toggle collapsed" data-toggle="collapse" data-parent="#accordion" href="#4">
 +
                        <div class="panel-title">
 +
                        <h3 style="padding: 0px; margin: 0px;">Parts construction</h3>
 +
<div class="row">
 +
  <div class="col-md-6">
 +
Part: <strong>P14.2</strong> (BBa_K1773006)
 +
  </div>
 +
  <div class="col-md-6">
 +
Written by: <strong>IR</strong><br />
 +
Performed by: <strong>ŠT</strong>, <strong>IR</strong>, <strong>MB</strong>, <strong>IS</strong>.
 +
  </div>
 +
</div>
 +
                        </div>
 +
                        </a>
 +
                    </div>
 +
                    <div id="4" class="panel-collapse collapse">
 +
                        <div class="panel-body">
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <h3><strong>About</strong></h3>
 +
  <p>pLac+cI.<br />P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.</p>
 +
  </div>
 +
</div>
 +
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <table class="table">
 +
    <thead>
 +
      <tr>
 +
        <th>Date</th>
 +
        <th>Materials</th>
 +
        <th>Experiment</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 +
      <tr>
 +
        <td>06 30</td>
 +
        <td>P10<br />P11</td>
 +
        <td>P10 and P11 transformation into DH5α.</td>
 +
      </tr>
 +
      <tr>
 +
        <td>07 01</td>
 +
        <td></td>
 +
        <td>Bacteria inoculation (4 minipreps).</td>
 +
      </tr>
 +
      <tr>
 +
        <td>07 09</td>
 +
        <td>P10<br />P11</td>
 +
        <td>
 +
Plasmids’ restriction (3 ug each).<br/>
 +
P10 with EcoRI and XBal (+FastAp).<br/>
 +
P11 with EcoRI and SpeI.<br/>
 +
Extraction from gel<br/>
 +
L10 and L11 are produced.<br/>
 +
Conc.: L11 – 2,4 ng/uL.
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 +
  </div>
 +
</div>
 +
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <h3><strong>Conclusions and Results:</strong></h3>
 +
  <p style="height: auto; margin: auto;">07 14 – all of the colonies are successful, except third colony.</p>
 +
  </div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
<!-- /MethodBox -->
 +
 +
<!-- MethodBox -->
 +
                <div class="panel panel-default" style="position: relative">
 +
                    <div class="panel-heading">
 +
                        <a class="accordion-toggle collapsed" data-toggle="collapse" data-parent="#accordion" href="#5">
 +
                        <div class="panel-title">
 +
                        <h3 style="padding: 0px; margin: 0px;">Parts construction</h3>
 +
<div class="row">
 +
  <div class="col-md-6">
 +
Part: <strong>P14.2</strong> (BBa_K1773006)
 +
  </div>
 +
  <div class="col-md-6">
 +
Written by: <strong>IR</strong><br />
 +
Performed by: <strong>ŠT</strong>, <strong>IR</strong>, <strong>MB</strong>, <strong>IS</strong>.
 +
  </div>
 +
</div>
 +
                        </div>
 +
                        </a>
 +
                    </div>
 +
                    <div id="5" class="panel-collapse collapse">
 +
                        <div class="panel-body">
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <h3><strong>About</strong></h3>
 +
  <p>pLac+cI.<br />P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.</p>
 +
  </div>
 +
</div>
 +
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <table class="table">
 +
    <thead>
 +
      <tr>
 +
        <th>Date</th>
 +
        <th>Materials</th>
 +
        <th>Experiment</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 +
      <tr>
 +
        <td>06 30</td>
 +
        <td>P10<br />P11</td>
 +
        <td>P10 and P11 transformation into DH5α.</td>
 +
      </tr>
 +
      <tr>
 +
        <td>07 01</td>
 +
        <td></td>
 +
        <td>Bacteria inoculation (4 minipreps).</td>
 +
      </tr>
 +
      <tr>
 +
        <td>07 09</td>
 +
        <td>P10<br />P11</td>
 +
        <td>
 +
Plasmids’ restriction (3 ug each).<br/>
 +
P10 with EcoRI and XBal (+FastAp).<br/>
 +
P11 with EcoRI and SpeI.<br/>
 +
Extraction from gel<br/>
 +
L10 and L11 are produced.<br/>
 +
Conc.: L11 – 2,4 ng/uL.
 +
        </td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 +
  </div>
 +
</div>
 +
 +
<div class="row">
 +
  <div class="col-md-12">
 +
  <h3><strong>Conclusions and Results:</strong></h3>
 +
  <p style="height: auto; margin: auto;">07 14 – all of the colonies are successful, except third colony.</p>
 +
  </div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
<!-- /MethodBox -->
  
  

Revision as of 21:08, 7 September 2015

About

pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.

Date Materials Experiment
06 30 P10
P11		 P10 and P11 transformation into DH5α.
07 01 Bacteria inoculation (4 minipreps).
07 09 P10
P11		 Plasmids’ restriction (3 ug each).
P10 with EcoRI and XBal (+FastAp).
P11 with EcoRI and SpeI.
Extraction from gel
L10 and L11 are produced.
Conc.: L11 – 2,4 ng/uL.

Conclusions and Results:

07 14 – all of the colonies are successful, except third colony.

About

pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.

Date Materials Experiment
06 30 P10
P11		 P10 and P11 transformation into DH5α.
07 01 Bacteria inoculation (4 minipreps).
07 09 P10
P11		 Plasmids’ restriction (3 ug each).
P10 with EcoRI and XBal (+FastAp).
P11 with EcoRI and SpeI.
Extraction from gel
L10 and L11 are produced.
Conc.: L11 – 2,4 ng/uL.

Conclusions and Results:

07 14 – all of the colonies are successful, except third colony.

About

pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.

Date Materials Experiment
06 30 P10
P11		 P10 and P11 transformation into DH5α.
07 01 Bacteria inoculation (4 minipreps).
07 09 P10
P11		 Plasmids’ restriction (3 ug each).
P10 with EcoRI and XBal (+FastAp).
P11 with EcoRI and SpeI.
Extraction from gel
L10 and L11 are produced.
Conc.: L11 – 2,4 ng/uL.

Conclusions and Results:

07 14 – all of the colonies are successful, except third colony.

About

pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.

Date Materials Experiment
06 30 P10
P11		 P10 and P11 transformation into DH5α.
07 01 Bacteria inoculation (4 minipreps).
07 09 P10
P11		 Plasmids’ restriction (3 ug each).
P10 with EcoRI and XBal (+FastAp).
P11 with EcoRI and SpeI.
Extraction from gel
L10 and L11 are produced.
Conc.: L11 – 2,4 ng/uL.

Conclusions and Results:

07 14 – all of the colonies are successful, except third colony.

About

pLac+cI.
P10 and P11 plasmids are used. pLac (P11) and repressor cI (P10) ligation.

Date Materials Experiment
06 30 P10
P11		 P10 and P11 transformation into DH5α.
07 01 Bacteria inoculation (4 minipreps).
07 09 P10
P11		 Plasmids’ restriction (3 ug each).
P10 with EcoRI and XBal (+FastAp).
P11 with EcoRI and SpeI.
Extraction from gel
L10 and L11 are produced.
Conc.: L11 – 2,4 ng/uL.

Conclusions and Results:

07 14 – all of the colonies are successful, except third colony.