Difference between revisions of "Team:Vilnius-Lithuania/Safety"

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<h2>Safety in iGEM</h2>
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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        <h3 class="text-heading">Our iGEM safety achievements</h3>
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          <p class="text-justify">We submitted "About our lab" and "About our project" surveys by June 26<sup>th</sup><br />We submitted "Final Safety Form" survey by August 28<sup>th</sup></p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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        <h3 class="text-heading">Safety training</h3>
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          <p class="text-justify">Before we started working on our project in the lab, we were given a comprehensive safety training, concerning issues such as working with hazardous chemicals, electrical equipment, biological materials and others. Training also included general laboratory safety – wearing protective clothing (lab coats) and gloves at all times, using goggles when necessary, taking care of various chemicals,  sterilizing and cleaning the lab, in order to prevent any possible contamination, handling the equipment.</p>
  
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[[File:Vilnius15_goggles.jpg|500px|thumb|Goggles when necessary!|center]]
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<h4>Safe Project Design</h4>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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        <h3 class="text-heading">Project safety</h3>
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          <p class="text-justify">When planning a project, the team of scientists should carefully think about how their achievements will be applied in real life by the society. Will the team construct a device that could be used by public? Or will the team create a laboratory system, which could be used by other scientists?</p>
  
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          <p class="text-justify">Our project is applicable for the second option. Coliclock is a system, which can be used by scientists in other laboratories, in other words, our users will be highly proficient people, possesing specific skills.</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h4>Safe Lab Work</h4>
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        <p class="text-justify">All the parts we used throughout our project are not toxic or dangerous for the environment. Throughout our project we used these strains:
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<li>BL21-DE3 E. coli</li>
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<li>JM109 E. coli</li>
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<h4>Safe Shipment</h4>
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<li>DH5α E.coli</li>
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<li>One Shot® TOP10 Chemically Competent E. coli</li>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 23:34, 12 September 2015

Our iGEM safety achievements

We submitted "About our lab" and "About our project" surveys by June 26th
We submitted "Final Safety Form" survey by August 28th

Safety training

Before we started working on our project in the lab, we were given a comprehensive safety training, concerning issues such as working with hazardous chemicals, electrical equipment, biological materials and others. Training also included general laboratory safety – wearing protective clothing (lab coats) and gloves at all times, using goggles when necessary, taking care of various chemicals, sterilizing and cleaning the lab, in order to prevent any possible contamination, handling the equipment.

Goggles when necessary!

Project safety

When planning a project, the team of scientists should carefully think about how their achievements will be applied in real life by the society. Will the team construct a device that could be used by public? Or will the team create a laboratory system, which could be used by other scientists?

Our project is applicable for the second option. Coliclock is a system, which can be used by scientists in other laboratories, in other words, our users will be highly proficient people, possesing specific skills.

All the parts we used throughout our project are not toxic or dangerous for the environment. Throughout our project we used these strains:

  • BL21-DE3 E. coli
  • JM109 E. coli
  • DH5α E.coli
  • One Shot® TOP10 Chemically Competent E. coli