Difference between revisions of "Team:Pasteur Paris/Week 2"

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<ol>
 
<ol>
−
   <li>Not I enzymatic  digest of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003. </li>
+
   <li style="text-align:left">Not I enzymatic  digest of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003. </li>
−
   <li>Gel migration of  the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003) </li>
+
   <li style="text-align:left">Gel migration of  the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003) </li>
 
</ol>
 
</ol>
 
<p align="center"><img src="https://static.igem.org/mediawiki/2015/0/0e/Igem-Pasteur_gel-1-week-2.png" alt="h" width="399" height="303" /></p>
 
<p align="center"><img src="https://static.igem.org/mediawiki/2015/0/0e/Igem-Pasteur_gel-1-week-2.png" alt="h" width="399" height="303" /></p>
Line 166: Line 166:
 
<p align="center"><strong><u>Figure </u></strong><strong><u>2</u></strong><u>:</u> Table of the  migration gel done previously (cf. figure 2).<u> </u></p>
 
<p align="center"><strong><u>Figure </u></strong><strong><u>2</u></strong><u>:</u> Table of the  migration gel done previously (cf. figure 2).<u> </u></p>
 
<ol>
 
<ol>
−
   <li>Storage at -20°C of BBa_808007,  BBa_808014, BBa_808011 (only for C2/C3) </li>
+
   <li style="text-align:left">Storage at -20°C of BBa_808007,  BBa_808014, BBa_808011 (only for C2/C3) </li>
 
</ol>
 
</ol>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
 
<ol>
 
<ol>
−
   <li>Plasmid  purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000. </li>
+
   <li style="text-align:left">Plasmid  purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000. </li>
 
</ol>
 
</ol>
 
<table border="1" cellspacing="0" cellpadding="0" width="764">
 
<table border="1" cellspacing="0" cellpadding="0" width="764">
Line 289: Line 289:
 
<p><strong><u>Figure 4</u></strong><u>:</u> Plasmid  purification for BBa_808007, BBa_808010, BBa_808011, BBa_808012, BBa_808013,  BBa_808014, BBa_316003, BBa_936000. </p>
 
<p><strong><u>Figure 4</u></strong><u>:</u> Plasmid  purification for BBa_808007, BBa_808010, BBa_808011, BBa_808012, BBa_808013,  BBa_808014, BBa_316003, BBa_936000. </p>
 
<ol>
 
<ol>
−
   <li>Plasmid  purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000. </li>
+
   <li style="text-align:left">Plasmid  purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000. </li>
 
</ol>
 
</ol>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>

Revision as of 00:05, 13 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 2

06/08 - 06/12

  1. Not I enzymatic digest of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.
  2. Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003)

h

 

Figure 1: Verification of the plasmid purification.

 

316003

808007

808010

808011

808012

808013

808014

936000

Theoretical size of insert(pb)

1061

4368

948

1011

1242

465

1506

885

Present inserts match to theoretical values

no

yes

C1/C2 yes
C3 no

yes

no

no

yes

no

 

Figure 2: Table of the migration gel done previously (cf. figure 2).

  1. Storage at -20°C of BBa_808007, BBa_808014, BBa_808011 (only for C2/C3)

 

  1. Plasmid purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000.

BB

clone 1

clone 2

clone 3

C (ng/µL)

260/280

260/230

C (ng/µL)

260/280

260/230

C (ng/µL)

260/280

260/230

808007

22.6

1.99

2.04

46.9

1.92

2.09

32.3

1.9

1.79

808010

13.5

1.85

1.39

57.1

1.95

2.17

31.4

1.86

1.84

808011

37.4

1.97

2

61.2

1.62

1.16

21

1.93

1.72

808012

30.2

1.93

1.94

54.9

1.91

2.12

21.1

1.94

1.81

808013

11.5

1.91

1.11

9.6

1.78

1.42

13.9

1.74

1.21

808014

33.9

1.89

1.62

24.3

1.96

2.14

14.4

1.81

1.38

316003

13.6

2.04

2.38

16.5

2.1

2.41

18.8

1.94

0.74

936000

18.2

1.98

1.19

13.1

1.97

0.93

11.9

2.16

1.18

Figure 4: Plasmid purification for BBa_808007, BBa_808010, BBa_808011, BBa_808012, BBa_808013, BBa_808014, BBa_316003, BBa_936000.

  1. Plasmid purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000.

 

h
 

Figure 5: Migration gel of digestion products.