Difference between revisions of "Team:Pasteur Paris/Week 2"

Line 122: Line 122:
 
<ol>
 
<ol>
 
   <li style="text-align:left">Not I enzymatic  digest of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003. </li>
 
   <li style="text-align:left">Not I enzymatic  digest of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003. </li>
−
   <li style="text-align:left">Gel migration of  the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003) </li>
+
   <li style="text-align:left">Gel migration of  the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013,  BBa_K808014, BBa_K936000, BBa_K316003). </li>
  
 
<p align="center"><img src="https://static.igem.org/mediawiki/2015/0/0e/Igem-Pasteur_gel-1-week-2.png" alt="h" width="399" height="303" /></p>
 
<p align="center"><img src="https://static.igem.org/mediawiki/2015/0/0e/Igem-Pasteur_gel-1-week-2.png" alt="h" width="399" height="303" /></p>

Revision as of 02:45, 13 September 2015

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 2

06/08 - 06/12




  1. Not I enzymatic digest of BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003.
  2. Gel migration of the Miniprep (BBa_K808007, BBa_K808010, BBa_K808011, BBa_K808012, BBa_K808013, BBa_K808014, BBa_K936000, BBa_K316003).

    3. h

    Figure 1: Verification of the plasmid purification.


     

    316003

    808007

    808010

    808011

    808012

    808013

    808014

    936000

    Theoretical size of insert(pb)

    1061

    4368

    948

    1011

    1242

    465

    1506

    885

    Present inserts match to theoretical values

    no

    yes

    C1/C2 yes
    C3 no

    yes

    no

    no

    yes

    no

    Figure 2: Table of the migration gel done previously (cf. figure 2).


  3. Storage at -20°C of BBa_808007, BBa_808014, BBa_808011 (only for C2/C3)

    4.  

  4. Plasmid purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000.

    5. BB

    clone 1

    clone 2

    clone 3

    C (ng/µL)

    260/280

    260/230

    C (ng/µL)

    260/280

    260/230

    C (ng/µL)

    260/280

    260/230

    808007

    22.6

    1.99

    2.04

    46.9

    1.92

    2.09

    32.3

    1.9

    1.79

    808010

    13.5

    1.85

    1.39

    57.1

    1.95

    2.17

    31.4

    1.86

    1.84

    808011

    37.4

    1.97

    2

    61.2

    1.62

    1.16

    21

    1.93

    1.72

    808012

    30.2

    1.93

    1.94

    54.9

    1.91

    2.12

    21.1

    1.94

    1.81

    808013

    11.5

    1.91

    1.11

    9.6

    1.78

    1.42

    13.9

    1.74

    1.21

    808014

    33.9

    1.89

    1.62

    24.3

    1.96

    2.14

    14.4

    1.81

    1.38

    316003

    13.6

    2.04

    2.38

    16.5

    2.1

    2.41

    18.8

    1.94

    0.74

    936000

    18.2

    1.98

    1.19

    13.1

    1.97

    0.93

    11.9

    2.16

    1.18

    Figure 4: Plasmid purification for BBa_808007, BBa_808010, BBa_808011, BBa_808012, BBa_808013, BBa_808014, BBa_316003, BBa_936000.


  5. Plasmid purification of BBa_316003, BBa_808010, BBa_808012, BBa_808013, BBa_936000.

 

h

Figure 5: Migration gel of digestion products.