Difference between revisions of "Team:Czech Republic/Protocols"
(→Permanent irreversible bonding) |
(→Soft lithography - PDMS molding) |
||
| Line 15: | Line 15: | ||
= Soft lithography - PDMS molding = | = Soft lithography - PDMS molding = | ||
| − | * | + | *Mix 40g of PDMS with 4g of curing agent |
| − | + | *Centrifuge the mixture at 3250 RPM for 3 minutes to remove the bubbles introduced during the mixing | |
| − | * | + | *Clean silicon master with air gun |
| + | *Wrap an aluminium foil around the edges of the silicon master to create a container | ||
| + | *Pour the PDMS mixture over the silicon master | ||
| + | *Cure the PDMS in an oven at 80°C for 2 hours | ||
| + | *Leave the PDMS to cool down | ||
| + | *Detach the PDMS carefully from the silicon master | ||
= Soft lithography - Bonding PDMS-Glass = | = Soft lithography - Bonding PDMS-Glass = | ||
Revision as of 15:03, 14 September 2015
Protocols
Contents
Purification
....
Restriction
...
GA
...
Soft lithography - PDMS molding
- Mix 40g of PDMS with 4g of curing agent
- Centrifuge the mixture at 3250 RPM for 3 minutes to remove the bubbles introduced during the mixing
- Clean silicon master with air gun
- Wrap an aluminium foil around the edges of the silicon master to create a container
- Pour the PDMS mixture over the silicon master
- Cure the PDMS in an oven at 80°C for 2 hours
- Leave the PDMS to cool down
- Detach the PDMS carefully from the silicon master
Soft lithography - Bonding PDMS-Glass
Preparation of the substrates
Glass slide
- Rinse the glass slide with acetone, isopropanol, and deionized water
- Dry the glass slide with air gun
- Dehydrate the glass slide on a hot plate at 120°C for 30 minutes
PDMS
- Slice the PDMS to individual PDMS replicas
- Drill holes for microfluidic inlets and outlets
- Clean the PDMS using a scotch tape
Air plasma treatment
- Place the glass slide and the PDMS replicas into a plasma cleaner, contact surfaces facing upwards
- Exhaust the atmosphere with a vacuum pump and wait until the pressure drops to 500 mTorr
- Activate the plasma for 2.5 minutes at Hi power
- Stop the plasma and open the valve to stabilize the pressure, continue immediately with the bonding phase
Permanent irreversible bonding
- Place the PDMS replica on the glass slide
- Place the bonded device in an oven at 80°C for 60 minutes.
- Seal the inlets and outlets with a scotch tape until use
...