Difference between revisions of "Team:UCLA/Notebook/Protein Cages/25 June 2015"

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Intro: Today we designed the primer sequence for our codon optimized cage sequence from Yates' lab. I initially researched the iGEM specifics for the prefixes and suffix they require, so that we knew how to design primers for the sequence. Additionally, another set of primers with NdeI (prefix) and XhoI (suffix) sites will be designed for protein expression within the pET22b vector, since this plasmid was used by Dr. Yates.
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Methods: We first spoke with Fasih to learn how to order gBlocks and to confirm our primer design methodology.

Revision as of 00:13, 26 June 2015

iGEM UCLA




Intro: Today we designed the primer sequence for our codon optimized cage sequence from Yates' lab. I initially researched the iGEM specifics for the prefixes and suffix they require, so that we knew how to design primers for the sequence. Additionally, another set of primers with NdeI (prefix) and XhoI (suffix) sites will be designed for protein expression within the pET22b vector, since this plasmid was used by Dr. Yates.

Methods: We first spoke with Fasih to learn how to order gBlocks and to confirm our primer design methodology.