Difference between revisions of "Team:UCLA/Notebook/Protein Cages/25 June 2015"
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Intro: Today we designed the primer sequence for our codon optimized cage sequence from Yates' lab. I initially researched the iGEM specifics for the prefixes and suffix they require, so that we knew how to design primers for the sequence. Additionally, another set of primers with NdeI (prefix) and XhoI (suffix) sites will be designed for protein expression within the pET22b vector, since this plasmid was used by Dr. Yates. | Intro: Today we designed the primer sequence for our codon optimized cage sequence from Yates' lab. I initially researched the iGEM specifics for the prefixes and suffix they require, so that we knew how to design primers for the sequence. Additionally, another set of primers with NdeI (prefix) and XhoI (suffix) sites will be designed for protein expression within the pET22b vector, since this plasmid was used by Dr. Yates. | ||
| − | Methods: We first spoke with Fasih to learn how to order gBlocks and to confirm our primer design methodology. | + | Methods: We first spoke with Fasih to learn how to order gBlocks and to confirm our primer design methodology. This is what we learned: |
| + | 1. select gBlocks gene fragments on the IDT (integrated DNA technologies) website | ||
| + | 2. name the order | ||
| + | |||
| + | 3. copy the gene from benchling into the sequence window | ||
| + | 4. test the complexity to make sure its good to go | ||
| + | 5. say no to all the questions asked by IDT | ||
| + | Notes: | ||
Revision as of 00:20, 26 June 2015
Intro: Today we designed the primer sequence for our codon optimized cage sequence from Yates' lab. I initially researched the iGEM specifics for the prefixes and suffix they require, so that we knew how to design primers for the sequence. Additionally, another set of primers with NdeI (prefix) and XhoI (suffix) sites will be designed for protein expression within the pET22b vector, since this plasmid was used by Dr. Yates.
Methods: We first spoke with Fasih to learn how to order gBlocks and to confirm our primer design methodology. This is what we learned: 1. select gBlocks gene fragments on the IDT (integrated DNA technologies) website 2. name the order
3. copy the gene from benchling into the sequence window 4. test the complexity to make sure its good to go 5. say no to all the questions asked by IDT Notes: