Difference between revisions of "Team:Stanford-Brown/Collaboration"

 
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   <title>Carousel Template for Bootstrap</title>
 
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         <h1>Collaborations<small> Working together!<small></h1>
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         <h1>Collaboration<small> Working across the Atlantic<small></h1>
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         <p>See our projects below</p>
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           <h2 class="featurette-heading">Our Vision<span class="small"> to create biological origami aka BiOrigami</span></h2>
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           <h2 class="featurette-heading">Collaboration<span class="small"> with the University of Edinburgh</span></h2>
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           <p class="lead">The <a href="https://2015.igem.org/Team:Edinburgh">University of Edinburgh iGEM 2015</a> team is creating a paper-based biosensor to detect purity of and contaminants in illicit drugs. In an effort to increase the applications of the proof-of-concept biosensor, the team wanted to incorporate microbial cellulose as an alternative to filter paper. This could decrease the cost of the biosensor as well as making the disposal easier. By using microbial cellulose that our team provided, the University of Edinburgh team was able to check the binding affinities of a cellulose binding domain (CBD) to the cellulose to see whether microbial cellulose-based biosensors are feasible. Our bioHYDRA project involved testing processed and unprocessed cellulose, and we sent the Edinburgh team a sample of each. This allowed the Edinburgh team to see if there is an advantage to the processing for their applications, and, since our future work includes expressing CBDs on spore coats, the processed sheets will be better for our applications as well. The data (see figure) shows that less protein dissociation occurred from the processed sheets.
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For the collaboration, the Edinburg IGEM 2015 used the BBa_K321357, which is an sfGFP fused to the cellulose binding domain CBDcex transcriptionally activated by Lacl. Since the CBDcex is attached to a fluorescence, sfGFP, the strength of the binding affinity is correlated with the absorbance level. The y-axis measures the absorbance in relative fluorescent unit while the x-axis is the time incubation. Although there is large error bar, there is a distinguishable difference between the binding affinity of the CBD onto the cellulose unprocessed and processed sheet. The high absorbance for the processed sheet indicates that the CBD binds more effectively than the unprocessed sheet. This experiment will be repeated in the near future.
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        <h2 class="featurette-heading">But how? <span class="small"> with the following projects below</span></h2>
 
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        <h2 class="featurette-heading">Polystyrene <span class="small">Engineering E. coli to produce polystyrene</span></h2>
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        <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p>
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      <h6>Copyright &copy; 2015 Stanford-Brown iGEM Team</h6>
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        <h2 class="featurette-heading">Polyhydroxyalkanoates <span class="small">Optimizing the production of biological PHA</span></h2>
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      <h2 class="featurette-heading">C.A.S.H.<span class="small"> Cellulose Associated Spore HYDRAS, or a biological contractile mechanism</span></h2>
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      <p class="lead">Based on work done by Chen et al. at Columbia university, we sought to employ the contractile properties of bacterial spores to use as a contractile mechanism for biOrigami.</p>
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      <h2 class="featurette-heading">CRATER <span class="small">Crisper Assited Transformation Efficient Reaction</span></h2>
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Latest revision as of 00:13, 18 September 2015

Carousel Template for Bootstrap

Collaboration Working across the Atlantic

Collaboration with the University of Edinburgh

The University of Edinburgh iGEM 2015 team is creating a paper-based biosensor to detect purity of and contaminants in illicit drugs. In an effort to increase the applications of the proof-of-concept biosensor, the team wanted to incorporate microbial cellulose as an alternative to filter paper. This could decrease the cost of the biosensor as well as making the disposal easier. By using microbial cellulose that our team provided, the University of Edinburgh team was able to check the binding affinities of a cellulose binding domain (CBD) to the cellulose to see whether microbial cellulose-based biosensors are feasible. Our bioHYDRA project involved testing processed and unprocessed cellulose, and we sent the Edinburgh team a sample of each. This allowed the Edinburgh team to see if there is an advantage to the processing for their applications, and, since our future work includes expressing CBDs on spore coats, the processed sheets will be better for our applications as well. The data (see figure) shows that less protein dissociation occurred from the processed sheets.

Generic placeholder image For the collaboration, the Edinburg IGEM 2015 used the BBa_K321357, which is an sfGFP fused to the cellulose binding domain CBDcex transcriptionally activated by Lacl. Since the CBDcex is attached to a fluorescence, sfGFP, the strength of the binding affinity is correlated with the absorbance level. The y-axis measures the absorbance in relative fluorescent unit while the x-axis is the time incubation. Although there is large error bar, there is a distinguishable difference between the binding affinity of the CBD onto the cellulose unprocessed and processed sheet. The high absorbance for the processed sheet indicates that the CBD binds more effectively than the unprocessed sheet. This experiment will be repeated in the near future.
Copyright © 2015 Stanford-Brown iGEM Team