Difference between revisions of "Team:KAIT Japan/Results"

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We purified Dronpa 145N and Dronpa 145K by His-tag purification.<br>
 
We purified Dronpa 145N and Dronpa 145K by His-tag purification.<br>
 
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<img src="https://static.igem.org/mediawiki/2015/3/3c/Kekka2.PNG"><br>
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We irradiated about 400 nm at purification of Dronpa 145N. It fluorescence green.<br>
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[SDS-PAGE]<br>
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We expressed Dronpa 145N, 145K and luciferase fusion protein at pcold vector in E.coli and obtained these proteins at His-tag purification. <br>
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Revision as of 03:50, 18 September 2015

Results


[Expression of Dronpa 145K and Dronpa 145N]
We were cloning Dronpa 145K and Dronpa 145N in E.coli. And we expressed these parts using pcold vector.



E.coli fluorescence when we irradiated about 400 nm.



[Purification of Dronpa 145N and 145K]
We purified Dronpa 145N and Dronpa 145K by His-tag purification.



We irradiated about 400 nm at purification of Dronpa 145N. It fluorescence green.



[SDS-PAGE]
We expressed Dronpa 145N, 145K and luciferase fusion protein at pcold vector in E.coli and obtained these proteins at His-tag purification.