Difference between revisions of "Team:KAIT Japan/Notebook"

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6/22~7/24:PCR(Dronpa145N,Dronpa145K)<br>
 +
7/25~7/28:Digested PCR Product of Dronpa 145N,K(6/22) and pCold Vector<br>
 +
7/29~7/31:Ligation (Dronpa145N +pCold Vector),and transformation the ligation product.<br>
 +
8/1:Get three clonies that brite under UV light, pick up these colonies and incubate in liquide culuture<br>
 +
8/2:Miniprep the clones and digest plasmids to confirm we get correct clones<br>
 +
8/3:purified plasmid(Dronpa 145N+pCold)<br>
 +
8/4:transform RFP to Ecoli<br>
 +
8/5:Glycerol stock clones that have plasmid(Dronpa 145N+pcold)<br>
 +
8/6:digested PCR Product of Dronpa 145N,K(7/29) by EcorⅠand PstⅠ<br>
 +
8/7:After miniprep RFP clone, Digested the plasmid (RFP+pSB1C3) by EcoRⅠand PstⅠ,Ligation (pSB1C3+Dronpa 145N,K),transform the ligation product to Ecoli<br>
 +
8/8~8/12:incubated clones (pCold+Dronpa145N) to exprese Dronpa 145N<br>
 +
8/13~8/14:digested plasmid (pCold+Dronpa145N) by EcoRⅠ and XhoⅠ<br>
 +
8/15~8/18:digested Dronpa 145K by EcorⅠand PstⅠ，inserted into pCold vector by ligase,transform it into Ecoli<br>
 +
8/19~8/22:ligation (pCold vector+Dronpa145Nand Dronpa145N),and transformation the ligation product.<br>
 +
8/23:digested purifyide plsmid to check insert，we are not able to get good result ,try again<br>
 +
8/24~8/25:digested  Dronpa145N by EcoRⅠ and, XhoⅠ,ligation (pCold vector+Dronpa145Nand Dronpa145N),and transformation the ligation product.<br>
 +
8/26:colony PCR<br>
 +
8/27:<br>
 +
8/28~8/29:<br>
 +
8/30:<br>
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8/31:<br>
 +
9/1:<br>
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9/2:<br>
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9/3:<br>
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9/4:<br>
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9/5:<br>
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9/6:<br>
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9/7:<br>
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9/8~9/10:<br>
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<br>
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<br>
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<br>
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Revision as of 04:42, 18 September 2015

Lab note


6/22~7/24:PCR(Dronpa145N,Dronpa145K)
7/25~7/28:Digested PCR Product of Dronpa 145N,K(6/22) and pCold Vector
7/29~7/31:Ligation (Dronpa145N +pCold Vector),and transformation the ligation product.
8/1:Get three clonies that brite under UV light, pick up these colonies and incubate in liquide culuture
8/2:Miniprep the clones and digest plasmids to confirm we get correct clones
8/3:purified plasmid(Dronpa 145N+pCold)
8/4:transform RFP to Ecoli
8/5:Glycerol stock clones that have plasmid(Dronpa 145N+pcold)
8/6:digested PCR Product of Dronpa 145N,K(7/29) by EcorⅠand PstⅠ
8/7:After miniprep RFP clone, Digested the plasmid (RFP+pSB1C3) by EcoRⅠand PstⅠ,Ligation (pSB1C3+Dronpa 145N,K),transform the ligation product to Ecoli
8/8~8/12:incubated clones (pCold+Dronpa145N) to exprese Dronpa 145N
8/13~8/14:digested plasmid (pCold+Dronpa145N) by EcoRⅠ and XhoⅠ
8/15~8/18:digested Dronpa 145K by EcorⅠand PstⅠ，inserted into pCold vector by ligase,transform it into Ecoli
8/19~8/22:ligation (pCold vector+Dronpa145Nand Dronpa145N),and transformation the ligation product.
8/23:digested purifyide plsmid to check insert，we are not able to get good result ,try again
8/24~8/25:digested Dronpa145N by EcoRⅠ and, XhoⅠ,ligation (pCold vector+Dronpa145Nand Dronpa145N),and transformation the ligation product.
8/26:colony PCR
8/27:
8/28~8/29:
8/30:
8/31:
9/1:
9/2:
9/3:
9/4:
9/5:
9/6:
9/7:
9/8~9/10: